燃煤型氟中毒对大鼠骨保护素及配体表达影响.pdfVIP

中国公共卫生2008年3月第24卷第3期 ChinJPublicHealthMar2008 Vo1．24No．3 327 文章编号：1001．0580【2008)03．0327．03 中图分类号：R 995 文献标志码：A 【论 著】 燃煤型氟中毒对大鼠骨保护素及配体表达影响 范伟 ，张华 ，喻茂娟 ，周琳业 ，韦艳 ，谢春 摘 要：目的 观察骨保护素(OPG)、骨保护素配体(OPGL)在燃煤型氟中毒大鼠骨组织中的表达水平，探讨燃 煤型氟中毒对大鼠骨保护素、骨保护素配体表达的影响。方法 以SD大鼠为研究对象。按体重均衡随机分为5组 (组内雌雄各半)：高氟组、高氟偏食组、低氟组、低氟偏食组、对照组。以氟病区煤烘玉米为主要饲料，复制氟中毒动物 模型。用免疫组织化学方法检测OPG、OPGL在大鼠胫骨干骺端的表达。结果 (1)各染氟组大鼠干骺端OPG表达 增高，与对照组比较差异有统计学意义(P0．05)。高氟组与低氟组比较，OPG表达增高。差异均有统计学意义(P 0．05)。(2)各染氟组大鼠干骺端OPGL表达增高。与对照组比较差异有统计学意义(P0．05)。高氟偏食组与高氟 组、低氟偏食组与低氟组比较，OPGL增高，差异有统计学意义(P0．05)。结论 过量氟造成骨与破骨活动均增强的 骨转换增高状态。氟可通过改变OPG和OPGL相对表达的比例影响骨吸收的程度。低钙偏食可促进氟骨症破骨吸 收增强。与过量氟对骨骼引起骨转换增强的作用具有同向性。 关键词：煤；氟中毒；骨保护素；骨保护素配体；骨 Effect of skeletal fluorosis caused by finoride in coal on OPG and OPGL ．AN w 。ZHANG HHa，YU Mao-juan，et a1． School ofPublic Health，Guiyang Medical College(Guiyang 550004，China) Abstract：0bjective To observe the exprea~ion of 0PG．OPGL in bone of fluorosis rats caused by coal burning and to explore the effects of skeletal fluorosis and osteoprotegerin and steoproteger in ligand．Methods SD rats taken as the experl— mental o4ects were randomly assorted into 5 groups(the number either female or male was the same in each group)：control group,high—dose fluoride group，high～dose fluoride group—emented with low calcium diet group，low—dose fluoride group，low—dose fluoride supplemented with low calcium diet group．All rats were fed with corn dried by burning coal，which came from the borough with high prevalence of fluoride，to establish the animal model of fluorosis．The tibia slices of chronic fluorosis rots were stained vith HE；0PG、0PGL in tibia metaphysis were observed by immunohistochemistry．Results COm— pared with control group，the number of 0PG increased in every test group and the difference was statistically