我国五大湖三角帆蚌群体ITS-1序列变异分析.pdfVIP

Lake Sci．(湖泊科学)，2008，20(2)：208—214 http：／／www．jlakes．org．E—mail：jlakes@niglas．ac．cI1 ~2008 byJournalofLakeSciences 我国五大湖三角帆蚌群体ITS一1序列变异分析 王建军 ，李家乐 ’ ，汪桂玲1白志毅 (1：上海水产大学农业部水产种质资源与养殖生态重点开放实验室，上海 200090) (2：上海高校水产养殖学E一研究院，上海 200090) 摘 要：对我国五大淡水湖：鄱阳湖、洞庭湖、太湖、巢湖、洪泽湖，鄱阳湖内包括进贤、余干、珠湖、都昌、湖口、永修，合 计 l0个群体三角帆蚌78个个体核糖体DNA基因转录间隔子ITS．1进行PCR扩增、序列测定，获得78条长度为430 bp的 同源序列．同源基因序列分析显示，五大淡水湖l0个群体三角帆蚌78条ITS—l序列片段，G+C的含量都明显高于A+T的含 量．鄱阳湖三角帆蚌的核苷酸多态性指数最高，而巢湖的核苷酸多样性指数最低．基于ITS．1序列片段的遗传距离表明，鄱阳 湖内六个点群体间遗传距离很小，在0 007l到0．0092之间．五大湖间三角帆蚌群体遗传距离较远，在0．0752到0．1381之间． ITS—l序列片段构建系统树显示，鄱阳湖6个群体聚在了一起为单独一支，并与巢湖群体亲缘关系相近．洞庭湖群体和洪泽湖 群体亲缘关系相近，单独为一支．太湖群体从鄱阳湖、巢湖群体分离开来，单独为一支． 关键词：三角帆蚌；转录间隔区；ITS．1；遗传多样性；亲缘关系 Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of Hyriopsis cumingii fr0m the Five Freshwater Lakes of China WANG Jianjun，LI Jiale，WANG GuilingBAI Zhiyi1 1．2 1 1 (1：Key Laboratory ofAquatic Genetic Resources and Aquacultural Ecology Certificated by the Ministry ofAgriculture，Shanghai Fisheries University,Shanghai 200090，P．R．China) (2：Aquaculture Division，E-Institute ofShanghai Universities，Shanghai 200090， ．China) Abstract：The internal transcribed spacer 1(ITS一1)was amplified from Hyriopsis cumingii in the five large lakes of China：Lake Poyang，Lake Dongting，Lake Taihu，Lak e Chaohu and Lake Hongze，and 430bp lengths of homological fragments of the ITS-1 was sequenced from 78 individuals of ten stocks．The homological fragm ent analysis showed that the average contents of G+C were obviously higher than that of A+ The nucleotide diversity of ITS of Lake Poyang was the highest．while that of Lake Chaohu was the lowest．The genetic distances among the six stocks from Lake Poyang were very meal,which was from 0．007 1 to 0．0092．The genetic distance among the five stocks from five large lakes were relatively far,which Was from 0．0752 to 0．1381．The phylogenetic tree ofITS一1 built with MEGA 3．1 showed that the six stocks from Lake Poyang clustered toget