嗅鞘细胞与聚乳酸-聚羟基乙酸共聚物材料的细胞相容性研究.pdfVIP

第 30 卷第5 期 第二军医大学学报 Vo l. 30 , No. 5 370 2佣8 年3 月 ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE Mar. 2∞8 文章编号:1删δ4归(2∞8 )05-0370-04 嗅黯细胞与聚乳酸·聚瓷基乙酸共聚物材料的细胞相容性研究 矫树生l ，李兵仓l ，陈建梅2 ，游 华1 e第兰军医大学大坪医院野战外科研究所第六研究室，创伤、烧伤与复合伤国家重点 实验室，重庆4创泊42;2南京军区福州总医院骨科，福州 35仪lOl ) 摘 要:目的探讨嗅鞠细胞(olfactory ensheathing cells , OECs) 与聚乳酸-聚瓷基乙酸共聚物[poly( DL-lactide-co-gly- col尬) ， PLGA] 材料的细胞相容性。方法将新鲜分离的 OECs 细胞悬液接种于 PLGA 膜上，然后用倒置相差显微镜、扫 描电镜观察细胞生长及细胞与材料的附着情况;MTT、流式细胞术等方法测定 OECs 的细胞增殖和细胞周期变化。结果 PLGA 膜上培养 OECs 的形态特征、增殖能力、细胞周期及倍体水平等与正常培养的 OECs 相比，均无显著性差异(P 0.05) 。结论 PLGA 生物材料与 OECs 相容性好，适于构建组织工程化人工神经。 关键词:嗅鞠细胞;聚乳酸·聚瓷基乙酸共聚物;细胞相容性;人工神经 中图法分类号: R3 18.08; R322.8; R329.24 文献标识码:A Cytocompatibility of biomaterial poly (DL-lactide-co-glycolide) with olfactory en- sheathing cells 1 1 2 1 JIAO Shu-sheng , LI Bing-cang , CHEN Jian-mei , YOU Hua (1 State Key Laboratory of Trauma , Bum and Combined Injury , Department of 6 , Research Institute of Surgery , Daping Hospital ，币lird Military Medical University , Chongqing 4α泊42; 2Fuzhou General Hospital of Nanji吨 Command ， Fuzhou 35仪lO 1, China) Abstract: Objective To investigate the cytocompatibility between poly (DL-lactide-co-glycolide) (PL- GA) and olfactory ensheathing cells (OECs) so as to found a basis to prepare an artificial nerve made of PLGA with OECs. Methods Freshly isolated OECs were seeded on PLGA membrane , and then their growth and ad- hesion to the membrane were observed at different time points by phase contrast inverted microscopy and scan- ning electron microscopy. The proliferation and the cell cycle of OEC