粪肠球菌和屎肠球菌临床分离株的毒力因子与耐药性分析.pdfVIP

热带医学杂志2008年5 第墨鲞 · 433 · 【文章编号】1672-3619(2008)05—0433~3 · 硕博专栏论著 · 粪肠球菌和屎肠球菌临床分离株的毒力因子与耐药性分析 吴敏，陈清 ，胡族琼，江凌晓，龙军 (南方医科大学公共卫生与热带医学学院流行病学系，广州 510515) 【摘要】 目的 检测临床分离肠球菌的耐药和毒力因子，比较粪肠球菌和屎肠球菌的耐药性和毒力特征。 方法 采用琼脂稀释法检测肠球菌对万古霉素(VAN)、四环素(TET)、环丙沙星(CIP)、红霉素(ERY)、复方新诺 明(SMZ)、替考拉宁(TEC)、克林霉素(CLI)的耐药性；采用微量板测定肠球菌的生物膜形成能力；观察肠球菌的 B溶血和明胶溶解结果，同时用PCR方法检测相应基因cylA和gelE。结果 粪肠球菌和屎肠球菌的耐药率分 别为O％～100％和3．23％～96．77％．后者对环丙氟哌酸、红霉素的耐药程度高于前者。13溶血试验阳性率为 19．23％，cylA基因阳性率为35．38％；明胶表型阳性率为21．54％，gelE阳性率为4O．O％，其中有46．15％(12／26) 阳性者未出现相应表型；生物膜形成检出率为36．92％；粪肠球菌3种毒力因子(表型和基因型)的阳性率均高于 屎肠球菌。结论 屎肠球菌耐药率高于粪肠球菌，粪肠球菌毒力因子阳性率高于屎肠球菌。 【关键词】肠球菌；p溶血；明胶酶；耐药；基因；生物膜 中圈分类号：R378．1 文献标识码：A Comparison of the Incidence of Virulence Determinants and Antibiotic Resistance between Clinical Enterococcus faecal~and Enterococcus faecium Strains WU Min，CHEN Qing ，HU Zu—qiong，JIANG Lin—xiao，LONG Jun (Department ofEpidemiology，Southern Medical Univer$ity，Guangzhou 510515，China) 【Abstract】 Objective The aim of this investigation was to compare the incidence of virulence determinants and antibiotic resistance between Enterococcus faecalis and E．faecium strains isolated from patients．Methods Antibiotic susceptibility testing Was performed using broth microdilation susceptibility tests to determine the Minimum Inhibition Concentration (MIC)． The phenotypic assay of gelatinase activity and 13-hemolysis were performed on agar plate supplemented with 3％gelatin and 5％rabbit blood． respectively． Virulence determinants were detected by PCR．The biofilm assay Was performed by using micmtiter plate．Results The antibiotic resistant percentage of E．faecalis and E． faecium strains were O％～100％and 3．23％~96．77％．respectively．