持续加力和间歇加力对骨髓基质干细胞增殖和分化的影响.docVIP

持续加力和间歇加力对骨髓基质干细胞增殖和分化的影响 陈俊良1，2，何 芸1，2，肖金刚1，2 (1西南医科大学附属口腔医院口腔颌面外科，四川省泸州市 646000；2西南医科大学口颌面修复重建和再生实验室，四川省泸州市 646000) 引用本文：陈俊良，何芸，肖金刚. 持续加力和间歇加力对骨髓基质干细胞增殖和分化的影响[J].中国组织工程研究，2017，21():1969-1973. DOI:10.3969/j.issn.2095-4344.2017.13.001 ORCID: 0000-0003-3997-5668(陈俊良) 文章快速阅读： 文题释义： 离心力：实验选择离心力作为机械外力刺激，其可控性较高，可以准确地控制加力时间、频率和大小。离心力可以在一定程度上反映骨髓基质干细胞受到机械力学刺激作用，虽然无法完全模拟体内牵张成骨时的实际受力情况，但可以提供一定的理论参考。 碱性磷酸酶和骨钙素：碱性磷酸酶是骨形成过程中的重要功能性酶之一，是成骨细胞早期分化的标志。骨钙素是由成骨合成和分泌的维生素K依赖性钙结合蛋白，在基质钙化后能明显上调表达，促进钙与羟基磷灰石结合及随后在骨基质的沉积，是成骨细胞成熟的标志。碱性磷酸酶、骨钙素的表达程度可以反映骨的形成过程。 摘要 背景：临床运用牵张成骨技术时常用的加力方式有2种：持续加力和间歇加力。但是，哪种方式更有利于新骨形成尚有争议。 目的：观察持续加力和间歇加力对骨髓基质干细胞分化及增殖能力的影响。 方法：密度梯度离心法分离培养兔骨髓基质干细胞，传至3代接种至6孔细胞培养板进行力学实验。空白组不作离心处理，静态培养；持续加力组每天加载离心4 h(1 100 r/min，相对离心力20×g)；间歇加力组每次加载离心力24 min(1 100 r/min，相对离心力100×g)，每天4 h内2次，2 h加力1次。Effect of continuous versus intermittent force on the proliferation and differentiation of bone marrow stromal stem cells Chen Jun-liang1, 2, He Yun1, 2, Xiao Jin-gang1, 2 (1Department of Oral and Maxillofacial Surgery, the Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China) Abstract BACKGROUND: In the process of distraction osteogenisis, continuous and intermittent force can be used. However, which one is more beneficial for bone formation is still controversial. OBJECTIVE: To investigate and compare the effect of continuous and intermittent force on the proliferation and differentiation of bone marrow stromal stem cells (BMSCs). METHODS: Rabbit BMSCs were obtained using density gradient centrifugation method. The passage 3 BMSCs were seeded onto 6-well cell culture plates for mechanical investigation. Then, the cells were randomly divided into three groups. Group A was a control group without any treatments. Group B was the continuous force group with a centrifugal force (1 100 r/min, relative centrifugal force