长期施用粪肥对农田土壤中细菌四环素抗性水平的影响.pdfVIP

安徽农业科学，Journal of Anhui A ，Sci．2008，36(14)：5944-5945，5947 责任编辑 金琼琼 责任校对 卢瑶 长期施用粪肥对农田土壤中细菌四环素抗性水平的影响 汪勇 ，2，林先贵z*，王一明z，闰淑珍 ，冯有智2 (1．南京师范大学生命科学学院，江苏南京 210046；2，中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室，江苏南京 210008) 摘要 的1考查集约化养殖产生的畜禽粪便直接施入农田造成的环境风险。【方法]以长期施用化肥的土壤为对照，通过小区定点试 验，研究长期施用新鲜鸡粪、新鲜猪粪后对土壤中四环素抗性细菌的影响。『结果]新鲜鸡粪、猪粪中四环素抗性细菌的数量及其占可 培养细菌总数比例均远远高于施肥前的基础土样。长期施用粪肥后，土壤中四环素抗性茵数量及其占可培养细菌总数比对照均有明 显增加，而且有随土壤深度的增加(0～20 cm)呈纵向增加的趋势。【结论]长期施用畜禽粪肥导致土壤中微生物抗生素抗性水平大幅增 加，对环境造成了一定的潜在风险。 关键词 粪肥；土壤；四环素抗性细菌 中图分类号 S141，1 文献标识码 A 文章编号 0517—6611(2008)14—05944—02 Effects of Long-term Application of Manure on Bacteria Resistance Level to Tetracycfine in Farmland Soil WANG Yong et al(College of Life Science，Nanjing Normal University，Nanjing，Jiangsu 210046) Abstract 【0bjective]The purpose of the research was to investigate the envl ronment risk of spreading the livestock and poultry manure produced bv l ntensive culture into farmland directly．『Method]With d1e soil applied chemic~fertilizer fer a long time as CK，the effects of long— term application of fresh chicken and pig manures on the tetracycline resistance bacteria in soil was studied through fixed-plot test．【Result]The amount of tetracycline resistance bacteria and its proportion accounting fer the total amount of cultured bacteria in fresh chicken and pig manure were greatly higher than that in the basic soil sample before manure application．And after long-term application of these manure the amount and its proportion accounting：for the total amount of cultured bacteria in soil were significantly higher than that in CK and this increase trended to vertieal increment with the soil depth of 0—20 cm．『Conclusion1 Long-term application of manure could lead the resistance level of the antibiotic antibiotics to I ncrease greatlv．which caused a potential risk to envi ronment． Key words Manure：Soil：Tetracycline resistance bacteria 随着集约化畜牧业和配方饲料工业的发展，抗生素作