鲍曼不动杆菌对β-内酰胺类抗生素的耐药性及耐药基因型研究.pdfVIP

· 230· J Clin Transfus Lab Med．July．2008，Vol 10，No．3 鲍曼不动杆菌对l3一内酰胺类抗生素的耐药性及耐药 基因型研究 肖燕 姚怡 朱雪明 周惠琴 【摘要】 目的 了解临床分离的鲍曼不动杆菌对 内酰胺类药物耐药性以及与 内酰胺酶耐药基因的关系。方法 采 用K—B纸片扩散法检测2O株鲍曼不动杆菌的耐药状况，用聚合酶链反应(PCR)方法检测6种口一内酰胺酶耐药基因(blaTEM、 bla 、bla。 一23、bla 、bla Dc、bla。 )并加以分析。结果 2O株鲍曼不动杆菌对头孢菌素类高度耐药，对碳青霉烯类大多 敏感。所有菌株均检出bla M、bla Dc基因，有18株携带blav 基因，其余基因均为阴性。结论 同时携带blaTEM、blaaDc和blav~ 基因是本院鲍曼不动杆菌对 内酰胺酶类抗菌药耐药的主要原因。 【关键词】 鲍曼不动杆菌 口一内酰胺类 耐药基因 【中图分类号】 R378．99 R969．3 【文献标识码】 A 【文章编号】 1671-2587(2008)03—0230—04 Study on the~-lactama Antibiotic Resistance and Genotype of[~-lactamases in Acinetobacter Baumanii XIAO Yan，YAO Yi，ZHU Xue—ruing，et a1．The second affiliated Hospital of Soochow University，Suzhou 215004 [Abstract] Objective To study the relationship between~[actama antibiotic resistance and resistant genes encoding lactamases in Acinetobacter baumanii of clinical isolates．Methods The antibiotic resistance was detected by kirby—bauber method in 20 strains of Acinetobacter baumanii．The~-lactamases resistant genes including blaTzM，blav~，blaoxA一23，blasuv， blaADcand blaudP were detected by polymerase chain reaction(PCR)．Results 20 strains of Acinetobacter baumanii were all with a high resistance tO cephalosporins while most of them were sensitive tO carbopenem—antibiotics．All of them carried blaTEM，blaADc and blav,M genes．18 strains of them carried blaVIM．Other genes were negative．Conclusions Carrying blaTEM，bla^Dc and blaviM genes was the main cause of口一lactama drug—resistance in Acinetobacter baumanii isolated from our hospita1． ’‘ [Key words] Acinetobacter baumanii 13-1actama antibiotics Antibiotic—resistant genes 鲍曼不动杆菌(Acinetobacter baumanii)是一 材料与方法 种条件致病菌，广泛存在于自然界和人体表面，由 1 材料 于对湿、紫外线和化学消毒剂有较强的抵抗力，可 1．1 菌