A Guide to Biosafety Biological Safety （生物安全与生物安全指南）.pdf

A Guide to Biosafety Biological Safety Cabinets Foreword This booklet was developed as a guide to biological safety cabinets and provides basic knowledge of biosafety and biological safety cabinets. The information presented is unbiased and generic in nature compiled with help from experienced microbiologists, engineers and safety enclosure users. I. BIohazards and BIosaFety LeveLs The word biohazard is a contraction of the words biological and hazard, and defined as: “an infectious agent, or part thereof, presenting a real or potential risk to the well-being of man, animals and / or plants, directly through infection or indirectly through disruption of the environment.” Biosafety Levels 1 through 4 were established by the Centers for Disease Control (CDC) and the National Institutes of Health (NIH) and are combinations of laboratory practices and techniques, safety equipment and facilities. All of these levels are appropriate for the biohazard posed by the agents used and for the laboratory activity. 2 Biosafety Level 1 Practices, safety equipment and facilities appropriate for work with defined and characterized strains of viable micro organisms not known to cause disease in healthy adult humans. The laboratory is not necessarily separated from the general traffic patterns in the building. Work is generally conducted on open bench tops using standard microbiological practices. Special containment equipment or facility design is neither required nor generally used. Laboratory personnel have specific training in the procedures conducted in the laboratory and are supervised by a scientist with general training in microbiology or a related science. A biological safety cabinet is generally not required for work involving these agents.