牛副流感病毒3型的分离鉴定.PDFVIP

微生物学通报 SEP 20, 2009, 36(9): 1384~1389 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 简 报 牛副流感病毒3 型的分离鉴定 * 刘 鹏 侯喜林 周玉龙 朴范泽 ( 163319) 摘 要: 从黑龙江省某牛场患有呼吸道疾病的病牛鼻液中分离到 1 株病毒, 该病毒能在MDBK 细 胞上增殖并产生特征性细胞病变, 对分离毒株进行理化特性、RT-PCR 鉴定及序列测定与分析, 结 果发现该病毒为RNA 病毒, 具有血凝性, 对乙醚、氯仿极其敏感, 不耐热, 对酸的抵抗力差; 分离 株与GenBank 中已发表的病毒株N 基因片段的同源性为82.6%~99.1%, 结果表明分离的病毒为牛 副流感病毒3 型毒株, 命名为 BPIV3 DQ1 株。根据 GenBank 上发表的牛副流感病毒3 型核衣壳 蛋白 N 基因序列, 设计合成了一对特异性引物, 能扩增 704 bp 的目的片段, 可以检测出 10 个 TCID50/100 μL 病毒核酸。 关键词: 牛副流感病毒3 型, 分离, 鉴定, RT-PCR Isolation and Identification of Bovine Parainfluenza Virus Type3 * LIU Peng HOU Xi-Lin ZHOU Yu-Long PIAO Fan-Ze (College of Animal Science and Technology, Heilongjiang August-First Land Reclamation University, Daqing, Heilongjiang 163319, China) Abstract: One virus strain was isolated from the nasal secretion of a bovine suspected to be respiratory tract disease at a beef farm in Heilongjiang province. The virus could grow well and produce typical cytopathic effect in MDBK cells. Bovine parainfluenza virus type3(BPIV3) has been identified by a series of systematic identification such as physicochemical features, RT-PCR, and sequence analysis. As a result, the virus is