2种剂型高效氯氰菊酯对德国小蠊酶活性影响.docVIP

2种剂型高效氯氰菊酯对德国小蠊酶活性影响[摘要] 目的: 探讨高效氯氰菊酯纳米乳剂和高效氯氰菊酯普通乳剂的施药周期对德国小蠊抗性相关酶活性的影响。方法: 微量点滴法对德国小蠊进行施药,一次性点药,以18 d为1个施药周期,测定点药后不同时间德国小蠊酶活性的变化趋势。分光光度法测定德国小蠊相关酶活性,采用轮廓分析研究高效氯氰菊酯纳米乳剂和高效氯氰菊酯普通乳剂的施药周期与酶活性的关系。结果: 高效氯氰菊酯纳米乳剂和高效氯氰菊酯普通乳剂对德国小蠊乙酰胆碱酯酶(AchE)、谷胱甘肽S-转移酶(GST)和P450-O脱甲基酶活性影响的轮廓均不平行(F值分别为174.025、1 017.969、1 364.271,均P0.001)。结论: 高效氯氰菊酯纳米乳剂与高效氯氰菊酯普通乳剂施药后对德国小蠊AchE、GST和P450-O脱甲基酶活性的影响趋势不同,轮廓既不平行也不重合。? [关键词] 高效氯氰菊酯; 纳米乳剂; 德国小蠊; 乙酰胆碱酯酶; 谷胱甘肽S-转移酶; P450-O脱甲基酶? [中图分类号] R184.39 [文献标识码] A [文章编号] 1671-7562(2010)04-0329-04? doi:10.3969/j.issn.1671-7562.2010.04.001?? Two kinds of formulations of beta-cypermethrin against German ?cockroaches enzyme activity?? FAN Xin-tian?1, SHEN Yan?2, SHEN Xiao-bing?2 (1. Nanjing Railway Centre for Disease Control and Prevention, Nanjing 210042, China; ? 2. School of Public Health, Southeast University, Nanjing 210009, China)?? [Abstract] Objective: To discuss the activity of application cycle of beta?cypermethrin nanoemulsions, beta?cypermethrin ordinary emulsion on resistance?related enzyme of German cockroaches. Methods: Micro?drip method for application German cockroaches with disposable medicine for 18 days of application as a cycle, activity trends was measured at different times after druging German cockroaches. Related activity of German cockroaches was measured by spectrophotometric determination. The relationship between application cycle of beta?cypermethrin nanoemulsion or beta?cypermethrin ordinary emulsion and enzyme activity was analysed with contour analysis. Results: The contours of acetylcholinesterase (AchE), glutathione S?transferase (GST)and cytochrom P450?O systemactivity of German cockroaches were not parallel between beta?cypermethrin nano emulsion group and beta-cypermethrin ordinary emulsion group (F values were174.025, 1 017.969 and 1 364.271, all P?0.001). Conclusion: The activity of AchE, GST and P450-O were in different trents after beta-cypermethrin nanoemulsion and beta-cypermethrin ordinary emulsion