住院儿童血培养330份标本结果研究.docVIP

住院儿童血培养330份标本结果研究【摘要】 目的 了解我院住院儿童使用双相瓶做血培养的结果及血液感染病原菌种类。方法 患儿血培养标本经孵育箱培养,分离所得菌株用法国梅里埃ATB Expression系统进行鉴定。结果 330例标本血培养中分离出细菌40株,总阳性率12.12%, 其中革兰氏阳性菌占67.5%,阴性菌占32.5%。双份血培养阳性率14.67%,一份血培养阳性率10.0%。结论 本院患儿血液感染病原菌以革兰阳性球菌为主,金黄色葡萄球菌22.5%,表皮葡萄球菌20.0%,其次为大肠埃希菌17.5%。双份血培养比单份血培养阳性率高4.67%,应尽量采取双份血做培养以提高阳性率。 【关键词】 血培养;病原菌 The Analysisfor 330 hospitalized children, blood culture results of specimens XU Yan-ru.Department of Laboratory MedicineofDalang Hospital of Dongguan City, Guangdong Province,Dongguan 523770,China 【Abstract】 Objective To understand our hospital for children to do two-phase blood culture bottle results and blood infections pathogens.Methods Children with blood culture specimens were incubated with box culture, strains were isolated from the French Merieux ATB Expression system identification. Results 330 cases of blood culture samples of bacteria were isolated from 40, the total positive rate of 12.12%, of which Gram-positive bacteria accounted for 67.5%, 32.5% negative bacteria. Double positive blood culture rate 14.67%, a positive blood culture rate of 10.0%. Conclusion The hospital blood infections in children with Gram-positive cocci, Staphylococcus aureus 22.5%, 20.0% Staphylococcus epidermidis,17.5% followed by Escherichia coli. Double than single blood culture positive blood culture rates were 4.67%, should be taken to double the blood culture done to improve the positive rate. 【Key words】 blood culture; pathogens 作者单位:523770广东省东莞市大朗医院检验科 血液细菌培养是对菌血症和败血症诊断和治疗的依据,为了解我院住院儿童使用双相血培养瓶做血培养的结果及病原菌种类,对我院2008年8月至2010年2月330例临床疑似菌血症和败血症的住院患儿采用法国生物-梅里埃公司生产的需氧双相血培养瓶进行血液培养。结果如下。 1 资料与方法 1.1 标本来源 330例血培养标本均来自本院住院患儿,其中150例患者在不同时间内取静脉血2次,180例患者取一次静脉血。 1.2 仪器与材料 双相血培养瓶、ATB Expression 自动细菌鉴定仪及细菌鉴定和药敏卡条均为法国-梅里埃公司生产;分离用培养基为郑州贝瑞特公司生产;肠道菌因子血清、沙门菌属和志贺菌属多价及单价血清购自卫生部兰州生物品研究所。 1.3 质量控制菌株 大肠埃希菌ATCC25922,金黄色葡萄球菌ATCC25923,铜绿假单胞菌ATCC27853。 1.4 方法 无菌手续取静脉血5 ml注入双相血培养瓶,摇匀、35℃每隔24 h观察肉汤中和琼脂上有无细菌生长。对有细菌生长者,均采用法国生物-梅里埃公司生产的ATB Expression系统进行鉴定及药敏试验。观察7 d