双黄连片对小鼠流感病毒及腺病毒感染保护作用.docVIP

双黄连片对小鼠流感病毒及腺病毒感染保护作用【摘要】 目的 观察双黄连片在实验动物体内抗流感病毒和腺病毒的作用，为临床用药提供试验依据。方法 建立流感病毒鼠肺适应株FM1感染小鼠模型和腺病毒ADV3株感染小鼠模型，分别灌胃给予高、中、低剂量双黄连片，设立病毒唑对照和正常对照组，观察各组小鼠的死亡数、测定小鼠存活时间、肺指数和肺组织流感病毒滴度。结果 双黄连片可明显降低流感病毒和腺病毒感染小鼠的死亡率，延长肺炎小鼠的存活时间,降低肺炎小鼠的肺指数；同时，双黄连片还能降低肺组织中流感病毒的滴度。结论 双黄连片在小鼠体内有明显抑制流感病毒和腺病毒的作用。 【关键词】 双黄连片；流感病毒；腺病毒；肺炎；肺指数 Antiviral activity of shuanghuanglian tablet against influenza A1 virus FM1 and adenovirus ADV3 in mice SHEN Si．yao，LIU Jun．hui，TIAN Yan．ru，et al.Department of Pathogenic Biology and Immunology，School of Medicine，Xian Jiaotong University，Xian 710061 China 【Abstract】 Objective To study the protective effects of shuanghuanglian tablet on influenza viral pneumonia and Adenoviral pneumonia in mice.Methods Viral pneumonia model established by influenza A1 virus FM1 and adenovirus ADV3 in mice.The mice were randomly divided into some groups:normal control group，shuanghuanglian tablethigh dose group(2．4 g/kg)，shuanghuanglian tabletmiddle dose group(1．2 g/kg)，shuanghuanglian tablet low dose group(0．6 g/kg)，virazole group(50 mg/kg)and normal control group.The mice were treated for 7 days,one time per day.Mortality wasobserved.Antiviral activities of shuanghuanglian tabletwere tested by survival time，pulmonary．index，and influenza virus tiler of lung in vivo.Results Compared with virazole group,shuanghuanglian tabeltcould obviously decrease mice mortality(P0．05),increased survival time(P0．05)and degraded pulmonary．index(P0．05)of influenza A1 virus FM1 and adenovirus ADV3 infection.Meanwhile，shuanghuanglian tablet could also decrease influenza A virus tiler of lung(P0．05).Conclusion Shuanghuanglian tablet has antiviral activity against influenza viral pneumonia and Adenoviral pneumonia in mice. 【Key words】 Shuanghuanglian tablet;Influenza A1 virus FM1;Adenovirus ADV3;Pneumonia;Pulmonary．index 双黄连是由双花(金银花)、黄芩和连翘三味中药提取制成的中药制剂，主要用于抗病毒、细菌感染和增强免疫力，治疗由病毒或细菌感染所致疾病，经过多年临床观察，疗效确切。双黄连片是双黄连制剂中便于携带、疗效可靠的剂型，是抗病毒和细菌感染的理想药物，具有较广泛的应用前景。为了进一步观察双黄连片对呼吸道病毒的作用，本实验试验模拟自然感染，将流感病毒和腺病毒用滴鼻