芹菜甲素对拟痴呆大鼠脑神经元保护机制探究.docVIP

芹菜甲素对拟痴呆大鼠脑神经元保护机制探究[摘要] 目的：探讨芹菜甲素对拟老年性痴呆（AD）大鼠脑神经元的保护机制。方法：给予D-半乳糖注射和三氯化铝灌胃建立慢性中毒型痴呆大鼠模型；在制备AD模型同时用芹菜甲素灌胃；用Y型迷宫试验观察其行为学改变；用免疫组化技术检测大鼠静脉血液中超氧化歧化酶(T-SOD)及丙二醛(MDA)活性的变化；用荧光标记法测定脑细胞内游离钙离子的浓度；采用流式细胞技术、激光共聚焦技术检测神经元细胞凋亡；并分别与对照组比较，观察芹菜甲素对大鼠神经元病变、学习记忆能力的影响。结果：模型组大鼠海马和皮质具有典型的细胞凋亡特征，T-SOD活性降低，MDA含量升高；而芹菜甲素治疗组海马和皮质神经细胞凋亡数减少，T-SOD活性升高，MDA含量降低(P0.05或P0.01)。结论：芹菜甲素具有明显抗氧化作用和抑制神经细胞凋亡作用，其改善AD大鼠学习记忆功能可能是通过多个环节整体治疗来实现的。 [关键词] 大鼠；老年性痴呆；芹菜甲素；神经保护 [中图分类号] R-332 [文献标识码]A [文章编号]1674-4721（2009）05（a）-011-03 A study on the mechanism of Donepezil’s protection in neuron of hippocampus in mice with Alzheimer’s disease ZHU Jiying1，WU Hongwei2，WANG Mingqin1，GAO Ying3，ZHOU Yongkun4 (1.The Second Affiliated Hospital, Shandong TCM University, Jinan250001，China; 2.Jinan medical insurance manage office, Jinan250002, China; 3.Jinan medical society, Jinan250031, China; 4.The first hospital attached to Shandong traditional Chinese medical University, Jinan250011, China) [Abstract] Objective: To study the mechanism of Donepezil’s protection in neuron of hippocampus in mice with AD. Methods: The mice were given to D-galactolipin and chlorinate aluminium by intragastric administration to establishchronic Alzheimer’s disease rat models, then Donepezil was given by intragastric administration. The behavioral abnormalities were investigated by Y type maze test. The expressions of T-SOD and MDA were measured by a immunohistochemistry technique. The performance and learning ability were observed. Results：Compared with model group, the performance records of learning and memory in Donepezil group were better (all P0.01), the activity of T-SOD were decreased clearly (all P0.05), and the expression of MDA was increased evidently (P0.01). Conclusion: Donepezil has functions of neuroprotective effect, improvelearning and memory ability of mice with AD，it may be involved in the mechanisms of multi approaches. [Key words] Mice;Alzheimer’s disease;Donepezil;Neuroprotection 现代医学研究表明：AD病理改变主要为tau蛋白