贲门癌组织中PTEN及VEGF表达及相关性探究.docVIP

贲门癌组织中PTEN及VEGF表达及相关性探究【摘要】 目的 通过对PTEN和血管内皮生长因子(vascular endothelial growth factor，VEGF)在贲门癌中表达的研究,结合临床病理特征,探讨PTEN和VEGF的临床意义及相关性。方法 应用免疫组织化学Ｓ-Ｐ法检测100例贲门癌组织及20例正常胃黏膜中PTEN和VEGF的表达。结果 贲门癌组织中PTEN蛋白表达率为34．00%(34/100),显著低于正常胃黏膜的表达95%(19/20)(P＜0．01),与组织分化程度呈正相关(Ｐ＜0．05),与淋巴结转移呈负相关(Ｐ＜0．01)。VEGF在贲门癌中的表达率为75．00%(75/100),显著高于正常胃黏膜的表达10．0%(2/20)( Ｐ＜0．01),与癌组织浸润深度呈正相关(Ｐ＜0．05),与淋巴结转移也呈正相关(Ｐ＜0．05),与组织分化程度呈负相关(Ｐ＜0．05)。PTEN在贲门癌中的表达与VEGF呈负相关(Ｐ＜0．01)。结论 联合检测PTEN、VEGF有助于提高贲门癌侵袭转移能力的评估,对贲门癌的预后判断具有重要的临床意义。 【关键词】 贲门癌;管内皮生长因子;免疫组织化学;PENT基因 Correlation between protein expression of PTEN and VEGF in human cardia carcinomaWANG Yong-lian，WANG Yi，WANG Zhong-min，et al． Dept of Thoracic Surgery，the Affiliated Hospital of Xinxiang Medical College，Weihui 453100，China 【Abstract】 Objective To investigate the expression of PTEN and vascular endothelial growth factor(VEGF) in cardia carcinom and the relationship between their expression．Methods The expression of PTEN and VEGF were detected using immunohistochemical S-P method． Results Among 100 cases of cardia carcinoma,34 showed positive staining of PTEN(34%),while among 20 cases of normal mucosa 19 cases showed positive staining of PTEN． The expression level of PTEN in high differentiated adeno carcinoma was higher than that in low differentiated adeno carcinoma． Also,the loss or decreased expression of PTEN was significantly correlated with lymph node metastasis and infiltrative deepness．In 100 cases of cardia carcinoma,75(75%)were positive staining of VEGF,while in 20 of normal stomach mucosa,only 2 showed positive staining of VEGF． The expression of VEGF was markedly correlated with infiltrative deepness and lymph node metastasis and differentiation．Conclusion Combined detection of PTEN and VEGF maybe helpful to evaluate prognosis and infiltrative capability of cardia carcinoma． 【Key words】 Cardia carcinoma；Vascular endothelial growth factors:Immunohistochemitry；PTEN genes 贲门癌是我国常见的恶性肿瘤，严重危害人类健康。PTEN基因是近年来发现的一个具有磷酸酶活性的抑癌基因,在维持细胞的增殖、分