原代类风湿关节炎成纤维样滑膜细胞及成熟细胞系生长特性比较.docVIP

原代类风湿关节炎成纤维样滑膜细胞及成熟细胞系生长特性比较[摘要] 目的 通过对原代类风湿关节炎成纤维样滑膜细胞及成熟细胞系生长特性进行比较，为类风湿关节炎（RA）研究用细胞提供多重选择。 方法 分离培养原代类风湿关节炎成纤维样滑膜细胞，传至第三代，与成熟细胞系镜下观察细胞形态。此外，以相同细胞数接种，观察细胞增殖速度。 结果 形态上原代类风湿关节炎成纤维样滑膜细胞与成熟细胞系有所差别，且增殖速度细胞系快于原代细胞。 结论 成熟细胞系可在一定程度上反映原代细胞的状态，并用于RA相关疾病研究。 [关键词] 原代类风湿关节炎成纤维样滑膜细胞；成熟细胞系；细胞形态；增殖速度 [中图分类号] R-33 [文献标识码] A [文章编号] 1674-4721（2012）07（b）-0008-03 Comparison of the growth characteristics between primary rheumatoid arthritis fibroblast-like synoviocytes and mature celline CAI Wenhong1 SUN Baodong1 ZHANG Baofeng2 YUE Ye3 CHENG Wenxiang3 LI Jinchao3 HU Yiping3 ZHANG Peng3 1.Rheumatology Department, Shenzhen People′s Hospital in Guangdong Province, Shenzhen 518039, China; 2.Nutrition Department, Shenzhen People′s Hospital in Guangdong Province, Shenzhen 518039, China; 3.Translational Medicine RD Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Guangdong Province, Shenzhen 518055, China [Abstract] Objective To compare the growth characteristics between primary rheumatoid arthritis fibroblast-like synoviocytes and mature celline to provide multiple choices for RA research. Methods Isolate and culture primary rheumatoid arthritis fibroblast-like synovial cells. After three passages cell morphology was observed with microscope. In addition, observe the speed of cell proliferation with the same number of cells inoculated. Results Some differences exist in the cytomorphology and the proliferation speed of celines was faster than primary cells. Conclusion The mature cells can reflect the status of primary cells to some extent, and have application in RA-related diseases research. [Key words] Primary rheumatoid arthritis fibroblast-like synovial cells; Mature cellines; Cytomorphology; Proliferation speed 全世界类风湿关节炎（rheumatoid arthritis，RA）受累人口众多，患病率为0.5%～1.5%，不同的地域及人群患病率存在一定差异[1-3]。我国RA患病率为0.32%～0.36%[4]。类风湿关节炎至今无法治愈，究其病因，可能与基因易感性、感染、年龄、性别、寒冷潮湿等相关[5]。目前治疗方法众多，有药物治疗、物理治疗、外科手术等，具有一定的缓解作用，但持续时间长，花费昂贵，对个人会造成巨大的经济负担及长期治疗所带来的身心负担。有研究称，RA发病2年后，