间接原子吸收光谱法测定鸡蛋中碘含量-食品科学.PDF

※分析检测 食品科学 2012, Vol. 33, No. 12 247 1 1 1 1 2 1 , 陈丽娟 ，冯 玲 ，罗 荻 ，柳 莎 ，姚程炜 ，黄宝美 * (1.绵阳师范学院化学与化学工程学院，四川 绵阳 621000 ； 2. 中国空气与动力发展中心设计与设备研究所，四川 绵阳 622653) 2+ + － 建立鸡蛋中微量碘测定的一种新方法——间接原子吸收光谱法。在抗坏血酸作用下，Cu 被还原为 Cu ，I 与Cu+ 反应生成CuI 沉淀，利用此原理，测定上清液中剩余铜的含量，从而间接求得鸡蛋中碘的含量。实验考察温度、 离心时间、反应时间、氧化剂用量及酸性介质对结果的影响，选取反应在30℃进行，离心时间 10min，质量浓度 1000 μg/mL 的抗坏血酸溶液0.80mL，酸性介质为pH4.0 的邻苯二甲酸氢钾缓冲溶液。该法操作简便，线性范围宽，检出限 低于 1.0 μg/mL ，相对标准偏差为0.58% 。该方法简单、快速，利用它测定食品中碘的含量，结果令人满意。 间接原子吸收光谱法；鸡蛋；碘 Determination of Iodine in Eggs by Indirect Atomic Absorption Spectrometry 1 1 1 1 2 1, CHEN Li-juan ，FENG Ling ，LUO Di ，LIU Sha ，YAO Cheng-wei ，HUANG Bao-mei * (1. College of Chemistry and Chemical Engineering, Mianyang Normal University, Mianyang 621000, China； 2. Institute of Facility Design and Instrumentation, China Aerodynamic Research Development Center, Mianyang 622653, China) Abstract：A new method to determine iodine in eggs by indirect atomic absorption spectrometry (AAS) was established. Based 2+ + + － on the reduction of Cu by ascorbic acid to Cu for precipitation between Cu and I , Cu residue in the supernatant was determined and calculated to obtain iodine content in eggs. The reaction was carried out using 0.8 mL of 1000 μg/mL ascorbic acid solution in pH 4.0 potassium hydrogen phthalate buffer at 30 ℃, and the centrifugation time was 10 min. This method was simple and