杏鲍菇液体菌种培养基的筛选及培养条件的探讨 - 中国农学通报.PDFVIP

中国农学通报 2011,27(7):452-456 Chinese Agricultural Science Bulletin 杏鲍菇液体菌种培养基的筛选及培养条件的探讨 马瑞霞，刘 慧 （安阳工学院生物与食品学院，河南安阳455000 ） 摘 要：采用液体摇瓶培养法，以菌丝生物量为主要指标，对杏鲍菇液体菌种培养基配方及培养条件进行 研究。试验表明，最适宜的培养基配方为：蔗糖3% ，玉米面3% ，麦麸3% ，酵母粉0.4% ，KH PO 0.25% ， 2 4 MgSO4 0.1%，ZnSO4 0.05%，CaSO4 0.05%，起始pH 为6~7。最适摇瓶培养条件为装液量80 mL/250 mL ，摇 床转速150 r/min ，接种量10% ，培养周期为6 天。 关键词：杏鲍菇；液体菌种；培养基；培养条件 中图分类号：S646.1+4 文献标志码：A 论文编号：2010-3106 StudyontheScreeningofLiquidSpawnMediumandExploreof CultureConditionsofPleurotuseryngii Ma Ruixia, Liu Hui (BiologyandFoodEngineeringCollege,AnyangInstituteofTechnology, Anyang Henan 455000) Abstract:The liquid spawn medium formula and culture conditions of Pleurotus eryngii in submerged culture with shake flask were studied according to the index of mycelia biomass. The results showed that the optimum formula for Pleurotus eryngii liquid spawn medium was as follows: sucrose 3%, corn meal 3%, wheat bran 3%, yeast powder 0.4%, KH PO 0.25%, MgSO 0.1%, ZnSO 0.05%, CaSO 0.05% and initial pH 6-7. The optimal 2 4 4 4 4 culture condition: Volume 80 mL/250 mL, shaking rate 150 r/min, inoculation rate 10% and 6 culture day. Keywords:Pleurotus eryngii; liquid spawn; medium; culture condition 0 引言 进行接种，简便快捷，利于管理，适宜规模化、工厂化生 [6-8] 杏鲍菇（Pleurotus eryngii），学名剌芹侧耳。菌肉 产 。为此，于2009—2010 年对杏鲍菇液体发酵培养 肥厚，质地脆嫩，在生物分类上属于担子菌亚门、层