IGF-Ⅰ在小儿中枢神经系统感染诊断中意义.doc

IGF-Ⅰ在小儿中枢神经系统感染诊断中意义【中图分类号】R725.1 【文献标识码】A 【文章编号】1672－3783(2011)10－0232－01 【摘要】目的：研究小儿中枢神经系统感染时血清及脑脊液中胰岛素样生长因子Ⅰ（IGF-Ⅰ）的变化及意义，为临床诊断提供参考。方法：2009年1月至2011年8月石河子大学医学院第一附属医院儿科住院治疗的中枢神经系统感染病例，其中化脓性脑膜炎34例、病毒性脑膜炎32例、对照组为正常患儿38例。腰椎穿刺收集脑脊液，作细胞计数和分类，检测葡萄糖、氯化物、总蛋白、白蛋白、免疫球蛋白IgG、IgM。用酶联免疫吸附试验双抗体夹心法测定血清及脑脊液标本IGF-Ⅰ含量。结果：1、对病毒性脑膜炎组、化脓性脑膜炎组、对照组的比较发现，脑脊液IGF-Ⅰ浓度差异有统计学意义（F=12.5，P＜0.05），血清IGF-Ⅰ浓度差异无统计学意义（F=2.1，P＞0.05）；2、脑脊液IGF-Ⅰ浓度与白蛋白、总蛋白、IgG、IgM成正相关（r=0.467、0.534、0.516、0.418，P＜0.05），与葡萄糖成负相关（r=-0.421，P＜0.05）。结论：脑脊液IGF-Ⅰ显著增高是化脓性脑膜炎的特征之一，影响脑脊液中蛋白质和葡萄糖的代谢，可以作为病毒性脑膜炎和化脓性脑膜炎的辅助鉴别指标。 【关键词】胰岛素样生长因子Ⅰ；化脓性脑膜炎；病毒性脑膜炎 The significance of diagnosis central nervous system infection of detecting IGF-Ⅰin children ZHANG Jing 【Abstract】Objective: To study the significance and the change of insulin-like growth factor Ⅰ (IGF-Ⅰ) of serum and cerebrospinal fluid in children with the central nervous system infections and to provide a reference for clinical diagnosis. Methods: The cases of central nervous system infection were hospitalization in Pediatrics, First Affiliated Hospital of Shihezi Medicine University from January 2009 to August 2011. The purulent meningitis cases were 34. The viral meningitis cases were 32.The control group included 38 normal children. The cerebrospinal fluid was collected by lumbar puncture.The cell count and classification, glucose, chloride, total protein, albumin, immunoglobulin IgG, IgM were detected.The IGF-Ⅰlevels of serum and cerebrospinal fluid were detected by enzyme-linked immunosorbent assay. Results: 1, Comparing to viral meningitis, purulent meningitis and control group,there was significantly difference to IGF-Ⅰ concentration of CSF (F = 12.5, P 0.05); 2, There was positive correlation to IGF-Ⅰ concentrations of cerebrospinal fluid and albumin, total protein, IgG, IgM (r = 0.467,0.534,0.516, 0.418, P 1.3 统计学处理 采用SPSS15.0统计分析软件进行数据处理,计量资料两组间比较采用t检验,多组间比较采用F检验， 多因素的相关性采用相关分析。? 2 结果 2.1 脑脊液细胞计数及分类结果 对病毒性脑膜炎组、化脓性脑膜炎组、对照组的比较发现，病毒性脑膜炎组浆细胞