不同脱钙液对牙齿牙周联合石蜡切片HE染色影响.doc

不同脱钙液对牙齿牙周联合石蜡切片HE染色影响【摘 要】 目的 探讨不同脱钙液对牙齿牙周联合切片HE染色质量的影响。方法 选用10%乙二胺四乙酸钠(EDTA)、复合酸(Plank-Rychlo脱钙液)和5%硝酸3种不同的脱钙液,分成A、B、C 3组用于犬和大鼠牙齿牙周组织联合石蜡切片脱钙,通过记录脱钙时间,观察HE染色效果,评价室温下(20℃～28℃)不同脱钙液对切片制作的影响。结果 A组脱钙液所制切片染色效果良好,但脱钙速度缓慢,所需时间较长;B组脱钙液脱钙速度快,所制切片染色好;C组脱钙液所制切片的染色强度和对比度均不如前两种脱钙液。结论 在制作牙齿牙周联合切片时,EDTA脱钙液和Plank-Rychlo脱钙液所制切片均能取得良好的染色效果,但EDTA脱钙液脱钙周期过长;Plank-Rychlo脱钙液脱钙均匀,速度快,更适合于临床速检工作;硝酸脱钙液组染色效果较差。 【关键词】 脱钙;牙齿;牙周组织;石蜡切片;HE染色 【Abstract】Objective To investigate the effect of different decalcifying fluid on HE color of teeth and periodontal tissue united sections.Methods 10%EDTA decalcifying fluid, mixed acid(Plank-Rychlo)and 5% nitric acid were divided into three groups: group A, group B and Group C, which were used to decalcify the united paraffin sections of tooth and periodontal tissue from dogs and rats. The effect of three kinds of decalcifying fluid on manufacture of united sections was evaluated at room temperature(20℃~28℃)by decalcification time and HE staining quality.Results Group A had a good effect of HE staining, but its decalcification time was too long. Group B had either high speed decalcification or high staining quality.Group C was the last choice with the regard of poor staining intensity and contrast.ConclusionEDTA decalcifying fluid and Plank-Rychlo decalcifying fluid can get a favorable staining effect, but has a long decalcification period,Plank-Rychlo decalcifying fluid is more suited to clinical quick examination due to well-distributed and high speed decalcification. Nitric acid decalcifying fluid has a poor staining effect. 【Key words】 Decalcification;Tooth;Periodontal tissue;Paraffin section;Hematoxylin/eosin staining 在涉及口腔组织病理的研究中,需要制作牙齿牙周组织联合切片进行诊断、观察和对比分析,但由于牙齿是全身硬度最高的组织,特别是牙釉质,其无机物成分羟基磷灰石[Ca10(PO4)6(OH)2] 含量占97%,硬度与石英相似;牙本质、牙骨质及牙槽骨坚硬致密、易碎难切,而牙髓、牙龈、牙周膜等软组织在切片过程中易受挤压、牵拉、分离变形甚至脱落。如果硬组织不能很好地进行脱钙处理,很难制得优良的切片并获得满意的染色结果。为更好地了解脱钙技术对牙齿牙周联合切片制作的影响,我们就室温条件下3种脱钙液的脱钙效果以及对切片HE染色质量的影响进行了对比探讨。 1材料与方法 1.1主要仪器 SAKURA Tissue-Teb vip全自动脱水机(日本);Leica石