平脉通颗粒对D-半乳糖所致亚急性衰老模型自由基代谢影响.doc

平脉通颗粒对D-半乳糖所致亚急性衰老模型自由基代谢影响[摘要] 目的 探讨平脉通颗粒对D-半乳糖所致亚急性衰老模型自由基代谢的影响。方法 Wistar大鼠60只,随机分为模型对照组、平脉通组。选用昆明种小鼠60只,随机分为模型对照组、平脉通组。实验结束后将大鼠处死,断头取脑,制成10%的组织匀浆,测定一氧化氮(NO)、脂褐素(LP)、丙二醛含量(MDA)及超氧化物歧化酶活力(SOD);收集小鼠血液测定SOD、谷胱甘肽过氧化物酶(GSH-Px)、MDA含量。结果 平脉通组与模型对照组比较,均有显著性差异(P0.05,P0.01),平脉通组中大鼠脑组织中NO含量显著增加,LP和MDA显著减少(P0.01),小鼠血清中SOD、GSH-Px的含量(P0.05,P0.01)明显提高。结论 平脉通颗粒使D-半乳糖所致亚急性衰老模型NO含量增加,调整自由基代谢,提高抗氧化酶的活力,抑制自由基对机体的损伤,改善机体衰老导致的各种病理变化。 [关键词] 平脉通颗粒; D-半乳糖; 衰老模型; 自由基; 大鼠; 小鼠 [中图分类号] R285.5 [文献标识码] A [文章编号] 1673-9701(2009)27-19-02 Effects of Pingmaitong Granules on Free Radical Metabolism of D-galactose-induced Subacute Aging Model PAN Guanghui CHEN Guohua MENG Xiangfeng SHEN Shouxiang Weifang People’s Hospital,Shandong Provice,Weifang 261041,China [Abstract] ObjectiveTo investigate the effects of Pingmaitong granules on free radical metabolism of D-galactose-induced subacute aging model. MethodsSixty Wistar rats were randomly divided into model control group and pingmaitong granule group,and 60 Kunming species mice were also randomly divided into model control group and Pingmaitong granule group. After the experiment was finished,Wistar rats were killed,and the brains were taken out to be prepared into 10% tissue homogenate for measuring the levels of NO,LP,MDA and SOD,and the blood from Kunming species mice was collected for measuring the levels of SOD,GSH-Px and MDA. ResultsPingmaitong granule group was significantly different from model control group(P0.05,P0.01). In pingmaitong granule group,the NO content of the Wistar rat brain was obviously increased,the levels of LP and MDA were decreased markedly(P0.01), and the levels of serum SOD and GSH-Px of Kunming species mice were obviously increased(P0.05,P0.01). ConclusionPingmaitong granules can increase the NO content of D-galactose-induced subacute aging model,adjustfree radical metabolism,raise the antioxidase activity,inhibit free radical damage to the body,and improve a variety of pathological changes caused by the