柔筋方对紧张型头痛大鼠张颌反射影响及机制探究.docVIP

柔筋方对紧张型头痛大鼠张颌反射影响及机制探究[摘要] 目的 探索柔筋方对紧张型头痛大鼠张颌反射的影响及作用机制。 方法 采用颈部双侧半棘肌局部注射三磷酸腺苷二钠注射液（ATP）制造紧张型头痛的动物实验模型。体重（254±28）g的SPF级Wistar大鼠40只，分为假手术组、模型组、柔筋方组和一氧化氮合成酶（NOS）抑制剂组，通过张颌反射定量分析紧张型头痛模型大鼠头区皮肤肌肉筋膜疼痛阈值及敏感性的变化。 结果 柔筋方组和NOS抑制剂组潜伏期延长（P ＜ 0.05或P ＜ 0.01），模型组疼痛阈值降低（P ＜ 0.01），柔筋方组和NOS抑制剂组疼痛阈值有下降趋势，但差异无统计学意义（P ＞ 0.05）。 结论 柔筋方可显著延长潜伏期，并减低疼痛阈值，降低头颈部肌筋膜敏感性，疗效机制与降低一氧化氮浓度有关。 [关键词] 紧张型头痛；大鼠；一氧化氮/一氧化氮合成酶；张颌反射 [中图分类号] R246.6 [文献标识码] A [文章编号] 1673-7210（2012）01（c）-0021-02 Research on influence and mechanism of Roujin Fang on jaw-opening reflex of tension-type headache rats WU Hongyun HU Zhiqiang Department of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Shandong Province, Ji’nan 250011, China [Abstract] Objective To study influence and mechanism of Roujin Fang on jaw-opening reflex of tension-type headache rats. Methods Models were established by injecting adenosine disodium triphosphate injection (ATP) in both semispinalis neck intramuscular. 40 Wistar SPF rats weighed (254±28) g were selected, and randomly divided into sham-operated group, model group, Roujin Fang group and nitric oxide synthase (NOS) group. Accroding to jaw-opening reflex (JOR), myofascial pain threshold and sensitivity were quantitatively measured. Results Latency in Roujin Fang group and NOS group were significantly longer than that in pre-injection (P ＜ 0.05 or P ＜ 0.01), pain threshold in model group was significantly lower (P ＜ 0.01) and pain threshold in Roujin Fang group and NOS group decreased, but without significant difference (P ＞ 0.05). Conclusion Roujin Fang can remarkably prolong latency, decrease pain threshold and reduce myofascial sensitivity, the mechanism is related to consistency of nitric oxide. [Key words] Tension-type headache; Rat; NO/NOS; Jaw-opening reflex 据2010年国内流行病学调查发布数据显示，目前在我国18~65岁人口中，紧张型头痛（tension-type headache，TTH）发病率为10.77%，约占原发性头痛的45.00%，是最常见的原发性头痛。随着生活习惯的改变，工作学习压力的增加，其发病率逐年增加。紧张型头痛发病机制尚不清楚，目前认为是颅周肌筋膜组织伤害性痛觉传入增强[1]，而内源