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Biotechnology Letters 22: 1383–1387, 2000. 1383 © 2000 Kluwer Academic Publishers. Printed in the Netherlands. Partial purification of nattokinase from Bacillus subtilis by expanded bed adsorption 1 1 1 1 2 Hong-Bo Hu , Shan-Jing Yao , Le-He Mei , Zi-Qiang Zhu Byung-Ki Hur 1Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, P.R. China 2Department of Biological Engineering, Inha University, Inchon 402-751, Korea Author for correspondence (Fax: 86-571-7951358; E-mail: yaosj@che.zju.edu.cn) Received 8 May 2000; Revisions requested 2 June 2000; Revisions received 30 June 2000; Accepted 3 July 2000 Key words: adsorption, expanded bed, nattokinase, thrombolytic activity Abstract Nattokinase was purified from unclarified Bacillus subtilis culture filtrate using an expanded bed with a purification factor of 8.2 and at a yield of 95%. The optimal pHs for adsorption and elution were 6.0 and 7.0, respectively. The expanded bed route shortened the process time by 8–10 h and increased the yield by 50% when compared with the conventional method. Introduction could be given orally used as an agent for thrombosis therapy though with caution. Intravascular thrombosis is one of the main causes The conventional isolation and purification of pro- of a wide variety of cardiovascular diseases. Fibrin teins from fermentation broths usually involves a aggregation in arteries causes this type of disease. complex sequence of clarification steps (such as cen- Thrombolytic therapy is now designed to achieve re-