美洲大蠊提取物的质量标准研究.pdfVIP

中药与临床 2013;4(5) ·20· Pharmacy and Clinics of Chinese Materia Medica ·炮制制剂· 美洲大蠊提取物的质量标准研究 1 2 1 1 1 张臻 ，宋芹 ，胡慧玲 ，何瑶 ，傅超美 [摘要] 目的：建立美洲大蠊提取物的质量标准。方法：采用TLC 法对美洲大蠊提取物进行薄层鉴别，用紫外 可见分光光度法测定总氨基酸的含量，用水分测定法对提取物的水分进行测定。结果：在TLC 色谱中均能显现出与 -1 2 对照药材相同的 个斑点；氨基酸含量测定方法的线性范围为 ～ ﹒ (r =0.9981) ，平均加样回收率为 5 0.008 0.022 mg mL 99.3%(RSD=2.46%) 。结论：所建立的方法可行，重复性好，可作为美洲大蠊提取物的质量控制标准。 关键词 美洲大蠊；提取物； ； [ ] TLC UV [中图分类号] R283.6 [文献标识码] A [文章编号] 1674-926X(2013)05-006-02 1 2 1 Study on the quality standard of the extracts of Periplaneta Americana/ZHANG Zhen , SONG Qin , HU Hui-ling , HE 1 1 Yao , FU Chao-mei //(1.Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan; 2.Chengdu University, Chengdu 611137,Sichuan) [Abstract] Objective: To establish the quality standard of the extracts of Periplaneta Americana . Method: TLC was used to identify the extracts of Periplaneta Americana . UV was used to detect the content of total amino acid and moisture determination method was used to detect the water content. Result: The sample and control medicine showed the same 5 spots in the TLC -1 -1 ﹒ ～