黑灵芝子实体水溶性多糖的理化性质及抗氧化活性的研究.PDFVIP

※基础研究 食品科学 2009, Vol. 30, No. 19 55 1 1 1 1 2 1, 鄢 嫣 ，张 汇 ，聂少平 ，陈 奕 ，陈 洁 ，谢明勇 * (1. 南昌大学食品科学与技术国家重点实验室，江西南昌 330047 ； 2. 江南大学食品科学与技术国家重点实验室，江苏无锡 214122) 目的：从黑灵芝中提取分离出粗多糖，纯化得到一种新型纯多糖，并对其纯度、理化性质及抗氧化活 性进行了研究。方法：采用Sevag 法脱除粗多糖中的游离蛋白，并分别采用乙醇分级沉淀以及Superdex-200 凝胶柱 层析法纯化多糖，经高效凝胶渗透色谱法检验纯度。分别对纯多糖的溶解性、热特性及光谱特性等多种理化性质 进行测定。最后分别采用苯甲酸钠法、FRAP(还原能力)法以及β- 胡萝卜素漂白法对黑灵芝多糖的羟基自由基清除 能力，总抗氧化能力以及对β- 胡萝卜素- 亚油酸氧化体系的抑制能力进行了分析。结果：分离出一种纯度较高的 多糖，且具有显著的抗氧化能力。结论：黑灵芝多糖具有显著的抗氧化活性。 黑灵芝；多糖；理化性质；抗氧化活性 Physico-chemical Characteristics and Antioxidant Activity of Polysaccharide from Ganoderma atrum Fruitbody 1 1 1 1 2 1, YAN Yan ，ZHANG Hui ，NIE Shao-ping ，CHEN Yi ，CHEN Jie ，XIE Ming-yong * (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China； 2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China) Abstract ：In order to investigate physico-chemical characteristics and antioxidant activity of polysaccharide from Ganoderma atrum fruitbody, a pure polysaccharide was isolated and purified sequentially using Sevag method, ethanol grade precipitation and Superdex-200 gel filtration chromatography. The purity of this polysaccharide was identified by HPLC. Solubility, thermal stability and spectral properties of this polysaccharide were also evaluated. Meanwhile, scavenging capability of hydroxyl free radicals, total antioxidant capability and suppression ability in β-carotene linoleic acid oxidative system were analyzed. It was concluded that polysaccharide from Ganoderma atrum has distinct antioxidant act