HPLC测定炒苍耳子配方颗粒中绿原酸及咖啡酸的含量.PDFVIP

HPLC 测定炒苍耳子配方颗粒中绿原酸及咖啡酸的含量 吴怀恩，韦志英，韦建华，蓝仁青，黄海燕(广西中医学院药学院，南宁 530001) 摘要：目的 建立同时测定炒苍耳子配方颗粒中绿原酸和咖啡酸的高效液相色谱法。方法 采用 Shim-pack VP-ODS 柱 (250 mm×4.6 mm ，5 µm) ；流动相为乙腈-0.1 %磷酸水溶液(10 ∶90) ；检测波长为 327 nm ；柱温为 35 ℃。结果 绿原酸 在 0.215 2~1.721 6 μg 内线性关系良好(r=0.999 9) ，咖啡酸在 0.022 96~0.114 8 μg 内线性关系良好(r=0.999 9) ；绿原酸、咖 啡酸平均加样回收率分别为 96.39%(RSD=1.48%)、100.02%(RSD=1.81%)。结论 所建立的方法简便、准确，可同时测定 炒苍耳子配方颗粒中绿原酸和咖啡酸的含量。 关键词：高效液相色谱法；炒苍耳子配方颗粒；绿原酸，咖啡酸 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2010)08-0744-03 Determination of Chlorogenic Acid and Caffeic Acid in Chaocangerzi Dispensing Granule by HPLC WU Huaien, WEI Zhiying, WEI Jianhua, LAN Renqing, HUANG Haiyan(College of Pharmacy, Guangxi Traditional Chinese Medical University, Nanning 530001, China) ABSTRACT: OBJECTIVE To develop an HPLC method for simultaneous determination of chlorogenic acid and caffeic acid in Chaocangerzi dispensing granule. METHODS Chlorogenic acid and caffeic acid were separated on Shim-pack VP-ODS (250 mm×4.6 mm, 5 μm) column and detected at 327 nm, 35 ℃ , with acetonitrile-0.1% phosphoric acid solution (10 ∶90) as mobile phase. RESULTS The method had good linear relationship within the range of 0.215 21.721 6 μg (r=0.999 9) for chlorogenic acid and 0.022 960.114 8 μg (r=0.999 9) for caffeic acid, respectively. The results of the average recovery were 96.39% (RSD=1.48%) and 100.02% (RSD=1.81%). CONCLUSION The method is simple, accurate and can be used to simultaneously determine chlorogenic acid and caffeic acid in Chaocangerzi dispensing granule. KEY WORDS: HPLC; Chaocangerzi dispensing granule; chlorogenic acid; caffeic acid 苍耳子系菊科植物苍耳 Xanthium sibiricum 公司) ；LG16-W 型高速离心机(北京医用离心机厂) ； Part. 的干燥成熟带总苞的果实，味辛、苦，性温， B3500S-MT 超声清洗仪(上海必能信超声有限公 有毒，归肺经。具有散风除湿、通鼻开窍的功效， 司) ；电热恒温鼓风干燥箱(上海跃进医疗器械厂) ； 用于治疗风寒头痛、鼻渊流涕、风疹瘙痒和湿痹 B