下一代测序纠错方法综述.PDF

第42卷 第3期 北 京 工 业 大 学 学 报 Vol.42 No.3 2016年 3月 JOURNAL OF BEIJING UNIVERSITY OFTECHNOLOGY Mar. 2016 下一代测序纠错方法综述 江育娥,黄 伟,林 劼 (福建师范大学软件学院,福州 350108) 摘 要:在面向下一代的测序技术中,前期纠错方法决定着测序的最终序列质量,因此成为当前研究热点,很多不 同的纠错方法被提出,对于这些方法的优缺点以及它们适合的应用,应该有清晰的认识和公正的评估. 介绍了现存 的各种下一代测序的纠错技术. 首先,从各种不同工具所基于的底层算法进行分析;接着,从测序不同平台来看读 段错误形成的特点;然后,从各种不同的测序应用角度来观察读段序列错误特征;最后,总结并阐述读段序列纠错 工具存在的问题和发展趋势. 关键词:下一代测序;序列分析;纠错方法;平台错误特征 中图分类号:TP319 文献标志码:A 文章编号:0254-0037(2016)03-0377-10 doi:10. 11936/ bjutxb2015060042 Error Correction in Preprocessing of Next-generation Sequencing JIANG Yu’e,HUANG Wei,LINJie (Faculty of Software,Fujian Normal University,Fuzhou350108,China) Abstract:To achieve accurate sequenced reads of high quality result,many error correction techniques were proposed. For all these error correction methods,it is essential to evaluate their characteristics and their fitted applications. Inthispaper,all error correction methodspublished inthefieldwerereviewed. First,fromtheunderlinedalgorithmspointofview,theseavailabletoolswereanalyzed. Second,fromthe NGS application platforms,error characteristics of short reads were presented. Third,from the different applications,the read error presentation in these places was reviewed. Finally,a conclusion was drawn and the possible future work for error correction of NGS was introduced. Key words: next-generation sequencing; sequence analysis; error correction; NGS application error characteristics DNA序列包含着种属的遗传、个体特征和产生 generation high throughput sequencing),或者称其为 的变异等重要的信息. 完整和完全地对基因进行解 下一代测序、新一代测序或二代测序 (next