正常胃粘膜及胃癌中P115、MIF表达的意义-第三军医大学学报.docVIP

胃癌组织和细胞中P115 、MIF的表达及意义 邓 玮，易永芬，闫田静，文 雪，屈玉玲 (重庆医科大学病理学教研室, 分子医学与肿瘤研究中心 重庆 400016) [摘要] 目的 观察胃癌组织和细胞中高尔基体转运蛋白P115和MIF的表达情况，探讨其在胃癌发生发展中的意义。方法: 用S-P、Western blot和RT-PCR检测P115及MIF在正常胃黏膜和胃癌组织，3种细胞株（正常胃黏膜上皮GES-1，不同分化程度的胃癌细胞株MKN-28，BGC-823）中的蛋白和mRNA表达情况。结果 P115和MIF在胃癌组织中的阳性表达率分别为73.3%、80%，在正常胃黏膜中的阳性表达率分别为40%、46.7%，胃癌组织中P115和MIF的表达明显高于正常胃黏膜（P 0.01 ) ，且P115的表达和MIF的表达呈正相关（r =0.433）；胃癌组织中P115、MIF的蛋白和mRNA表达水平明显高于正常胃黏膜组织（P 0.0）（P 0.0）（P 0.05）Significance Of P115, MIF expression in human gastric carcinoma tissues and cell lines Deng Wei, Yi Yongfen, Yan Tianjing, Wen Xue, Qu Yuling (Department of Pathology, Chongqing Medical University, Chongqing 400016) [Abstract] Objective To investigate the expression of P115, MIF and their roles in the tumorigenesis and progression of gastric carcinoma. Methods To detect the experession of P115 , MIF mRNA and protein on human normal gastric mucosa and gastric carcinoma tissues, three cell lines(normal gastric epithelial cell line GES-1, differentiation of gastric carcinoma cell lines MKN-28、BGC-823) by SP method , RT-PCR and Western-blot. Results The positive rates of P115 and MIF were 73.3%、80%，respectively, in gastric carcinoma ; and 40% 、46.7%，respectively in the normal gastric mucosa. The positive rates of P115 and MIF in the gastric carcinoma were higher than in the normal gastric mucosa（P 0.01 ). However, P115 expression had a positive correlation with MIF（r =0.433）. The relative levels of p115、MIF protein and mRNA in the gastric carcinoma were higher than in the normal gastric mucosa（P 0.01 ). Immunocytochemistry, Western blot, RT-PCR analysis showed the level of P115 , MIF protein and mRNA in gastric carcinoma cell lines MKN-28、BGC-823 were higher than in the normal gastric epithelial cell line GES-1（P 0.01 ).However, the expression of P115, MIF in poorly-differentiated cell line was higher than in the well-differentiated cell line（P 0.05）. Conclusion The excessive expression of P115 and MIF may play synergetic roles i