电针刺激对心脏手术病人心肌热休克蛋白mRNA基因表达影响.docVIP

电针刺激对心脏手术病人心肌热休克蛋白mRNA基因表达影响[摘　要]　观察电针刺激对心脏手术病人心肌细胞热休克蛋白mRNA基因表达的影响。28例ASD病人,随机分为针麻组(组Ⅰ,n=6),针刺加全麻组(组Ⅱ,n=10),和全麻组(组Ⅲ,n=9),于转流前10 min,停转流,停转流30 min,停转流1 h测定CK?MB。结果:①3组病人停转流和转流后1h CK?MB均较转流前明显增加,但组Ⅲ的升幅明显高于组Ⅰ和组Ⅱ;②针刺组HSP70mRNA表达高于对照组(P0.05)。因此针刺有增加缺血心肌细胞HSP70mRNA表达作用。 [主题词]　电针;心脏外科手术;热休克蛋白质类/代谢;RNA,信使/代谢 Effects of Electroacupuncture on Myocardial Cellular HSP70mRNA Gene Expression in Patients Undergoing Cardiac Surgery Wang Xiangrui?1,Zhang Tengfei?2,Ma Shuliang?1,et al(1. Renji Hospital Affiliated to Shanghai Second Medical University,Shanghai 200127;2. Teaching and Research Section of Biochemistry,Shanghai Second Medical University) [Abstract]　Purpose　To observe the effect of electroacupuncture on myocardial cellular HSP70mRNA gene expression in patients undergoing cardiac surgery(ASD).Methods　28 ASD patients were randomly divided into 3 groups,i,e,acupuncture anesthesia group(groupⅠ,n=6),acupuncture combined with general anesthesia group(group Ⅱ,n=10) and general anesthesia group(groupⅢ,n=9).The sample from left atrium at 10 min before CPB,stopping CPB,30 min and 1 h after stopping CPB.Extration of total RNA from myocardial tissue using Reagent Kit.The concentration of mRNA was measured through RT?PCR and CK?MB was detected.Results　After CPB CK?MB in the three groups was significantly higher than the value of 10 min before CPB,and CK?MB in the group Ⅲ was remarkedly higher than the group Ⅰ and Ⅱ;HSP70mRNA expression was found to be higher in the group Ⅰ than that of group Ⅲ at 30 min and 1 h after stopping CPB.Conclusion　Electroacupuncture can induce the expression of HSP70mRNA in ischemic myocardial tissue of patients undergoing cardiac surgery. [Key words]　Electroacupuncture;Heart Surgery;RNA,Massenger/metab;Heat?Shock Proteins/metab;Myocardium/metab 体外循环心脏手术术后并发症的发生率与术中心肌缺血、缺氧的保护有明显关系[1]。除采用低温、不同成份灌注液的冠脉灌注等方法外,近年发现机体有自身的保护机制,心肌缺血、缺氧后诱导心肌内应激蛋白增加,从而增强机体抗御缺血的能力。笔者发现针刺麻醉病人术后恢复快,并发症少。为探讨针刺对心脏功能的调节作用[2],本文观察针刺对心脏手术病人心肌细胞热休克蛋白mRNA基因表达的影响,初步分析两者之间的相