纹状体内微量灌注Tourette综合征患者血清对大鼠刻板行为影响.docVIP

纹状体内微量灌注Tourette综合征患者血清对大鼠刻板行为影响【摘 要】 目的:根据Tourette综合征(Tourette syndrome,TS)发生的自身免疫机制建立TS大鼠实验动物模型。方法:采用酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测符合DSM-IV诊断标准的TS患者血清中的抗神经抗体滴度;通过微量渗透泵将含有高浓度(0.29±0.06 U/L)抗神经抗体的TS患者血清,以0.5 μL/h的速度缓慢注入大鼠纹状体内。在微量灌注结束后1、7、14、21天观察大鼠刻板动作行为,包括啮咬(用牙齿咬笼子,嚼木屑,无目的咀嚼)、将前爪举到嘴边或面部、舔(不包括理毛)、理毛行为、摇头、摇动爪子、用后腿站立及阶段性的发声,将所观察到的各项动作行为计数及其总和作为评估刻板运动的指标。结果:选取抗神经抗体浓度最高的8例TS患者血清和浓度最低的8例对照血清用于造模实验[(0.29±0.06 )U/L vs.( 0.10±0.04) U/L,P0.001]。向大鼠纹状体内微量灌注TS病人血清后,大鼠的刻板行为增加[(37.2±7.1 )vs.(106.3±11.7),P0.001];灌注TS儿童血清的大鼠的刻板行为比灌注正常儿童血清的刻板行为增加[(106.3±11.7 )vs.(31.2±6.2),P0.001]。结论:向大鼠纹状体内微量灌注含有高浓度抗神经抗体的TS病人血清,可以使大鼠的刻板行为增加。 【关键词】 Tourette 综合征;动物模型;刻板行为;抗神经抗体 中图分类号:R748,R749.94 文献标识码:A 文章编号:1000-6729(2010)001-0069-05 doi:10.3969/j.issn.1000-6729.2010.01.省略 【Abstract】 Objective:To establish animal model on the basis of the autoimmune etiology for a subset of cases of Tourette syndrome.Methods:Blood samples were drawn from patients with TS(by DSM-IV)and were sent for further enzyme-linked immunosorbent assays(ELISA)to a laboratory.Eight serum samples with the highest concentration of anti-neural antibody were selected for TS model group,and 8 serum sampled with the lowest concentration of anti-neural antibody were selected for the control group.Osmotic mini pump filled with undiluted TS or control serum were microinfused into the rat striatum at a rate of 0.5 μl /h for 72 h.Stereotypic movements were recorded at 1 d,7 d,14 d and 21 d after microinfusion.Several categories of stereotypy including bites(teeth touching the cage,wood chips,vacuous chewing or other objects except the body),taffy pulling(raises of the forepaw to the mouth and face),self-gnawing,licking not associated with grooming,grooming,head shaking,paw shaking,rearing and episodic utterances(EU)were recorded.Results:The anti-neural antibody serum concentration used for TS model was(0.29±0.06) U/L,and that used in control group was (0.10±0.04) U/L.After infusion of TS sera,stereotypic b