大鼠梗阻性黄疸时肾皮质微循环变化及意义 - 第三军医大学学报.doc

大鼠梗阻性黄疸时肾皮质微循环变化及意义 秦琦瑜1，李勇2，张庆富3，陈进军1，范立侨2，王车江3，赵群2（河北医科大学：1第一医院肝胆外科，2第四医院外三科， 3第一医院烧伤整形科，石家庄050031） 摘要：目的 探讨梗阻性黄疸大鼠肾皮质微循环变化及意义。方法 48只SD大鼠分为假手术对照组（SO组）；胆总管结扎组（BDL组），每组分3、7、10d三个时相。应用WX-9型微循环显微镜观测各组大鼠肾皮质微血管管径和密度变化及微血管周围的渗出情况。应用LISCA激光多普勒组织灌流图像仪观测各组大鼠肾皮质血液灌流量的变化。结果 胆总管结扎后，肾皮质微血管收缩；血液灌流值减小；7、10d组微血管数量减少；且随胆道梗阻时间延长变化更加明显（P＜0.0）d组微血管周围出现渗出。结论 梗阻性黄疸可引起大鼠肾皮质微循环障碍，血液灌流量减少。 关键词：黄疸；梗阻性；肾脏；微循环 中图分类号：R657.4 文献标识码：A Alteration and Significance of Renal Cortex Microcirculation in Obstructive jaundice Rats QIN Qi-yu1,LI Yong2,ZHANG Qing-fu3,CHEN Jin-jun1,FAN Li-qiao2,WANG Che-jiang3,ZHAO Qun2（1Department of the liver and the gallbladder surgery, The 1st Hospital.2Department of surgery, The 4th Hospital.3Department of burn and plastic surgery, The 1st Hospital of Heibei Medical University, Shijiazhuang 050031,China） Abstract Objective To observe the lteration and Significance of renal cortex microcirculation in obstructive jaundice rats. Methods 48 SD rats were randomly divided into two groups: sham operation group(SO), common bile duct ligation group(BDL).and observed at 3、7、10days. To observe the renal cortex microcirculation( the caliber ,density and effusion of the capillary) by the WX-9 microscope. and to observe the alteration of the renal cortex hemoperfusion by LISCA laser Doppler Hemoperfusion image instrument in different group. Results After Common bile duct was liqated, The caliber of capillary contracted;The amount of hemoperfusion was decreased;The amount of capillary was decreased at the 7 and 10days.They were decreased more significantly along with the time(P0.05).To different observe time had 8 he 基金项目：河北省科技支撑计划项目（072761964） Projects in the Science Technology Pillar Program of Hebei Province（072761964） E-mail:qinqiyu@ 通信作者：李 勇，E-mail: li_yong_hbth@126.com 通信作者：张庆富，E-mail: zhangqfdoct@ effusion of capillary were observed at 7 and 10 days. Conclusions Obst