白藜芦醇对糖尿病小鼠抗应激的实验研究.pdf

· 2014 年第27 卷第12 期 方 药 药理研究 白藜芦醇对糖尿病小鼠抗应激的实验研究 △ 马 毅，徐 丽 ，曹军平，李 霞，孙慧萍，李冬梅 武警总医院，北京 100039 ［摘 要]目的：探讨白藜芦醇对糖尿病小鼠抗应激能力的影响。方法：四氧嘧啶腹腔注射造成小鼠糖尿 病模型，分为白藜芦醇高、中、低剂量组（20、10、5mg/kg），模型组，正常组。腹腔注射给药7天后，分别通过爬 绳、负重游泳、耐缺氧、耐高/低温实验，评价白藜芦醇对小鼠应激能力的影响。结果：白藜芦醇各剂量组均可 显著延长糖尿病小鼠的爬绳时间(5.2～6.9min)、游泳耗竭时间(17.9～20.0min)和常压耐缺氧下的生存时间 （15.9～16.7min），与模型组比较有统计学意P ＜0.05）；白藜芦醇高义（ 、中剂量组可明显增强小鼠耐高温、耐 低温能力（P ＜0.05），降低死亡率（P ＜0.05）。结论：白藜芦醇能提高糖尿病小鼠的抗应激能力。 ［关键词]糖尿病；白藜芦醇；小鼠；抗应激 ［中图分类号］R285.5［文献标识码］A ［文章编号］1004-6852(2014)12-0015-03 Experimental Study of Resveratrol on Anti-stress Ability of Diabetic Mice △ MA Yi, XU Li , CAO Junping, LI Xia, SUN Huiping, LI Dongmei General Hospital of Armed Police Forces, Beijing 100039, China Abstract Objective: To discuss the influence of resveratrol on anti-stress ability of diabetic mice. Methods: Diabetic model was established by intraperitoneal injection of alloxan, and the models were divided into high, mod- erate and low dose groups of resveratrol (20, 10 and 5 mg/kg), the model group and normal group. In seven days af- ter peritoneal injection, the influence of resveratrol on mice was assessed by rope climbing, loaded-swimming, hy- poxia tolerating, heat-resistant/cold-resistant experiment. Results: Resveratrol of different doses could effectively prolong the time of rope climbing (5.2~6.9)min loaded-swimming time (17.9~20.0) min and survival time (15.9~16.7) min of hypoxia tolerating of diabetic mice, the differences had statistical meaning compared with the control group(P＜0.05); the ability of heat