基础研究活血化瘀汤对骨折早期缺氧诱导因子1及.pdfVIP

216 2005 4 18 4 China J Orthop Trauma, Apr. 2005, Vol. 18, No. 4 1 张俐, 叶俊材, 陈伯仪, 王和鸣, 张安桢 ( , 350003) : 1(H IF1) mRNA ( VEGF) : SD 72 , , ( , n = 36) ( , n = 36) , 1235714 d , 05 cm TRPCR , HIF1 mRNA VEGF :2 d, HIF1mRNA ; 1 d 2 d, HIF1mRNA ( P 001) ; 35714 d VEGF ( P 001) ; : HIF1mRNA VEGF H IF1 VEGF ; ; 1mRNA; Experimental research on the influence of Huoxue Huayu decoction( ) to HIF1 mRNA and VEGF expression during early fracture healing ZH A N G L i, YE Juncai , CH EN B oyi , WA N G H em ing , ZH A N G A nz hen . Dep ar tment o Or thop aedics and Tr aumatology o F uj ian Univ er sity o T CM , F uj ian Fuz hou , 350003, China A stract O jective:To explore the expression of hypoxic induct ive factor1mRNA (H IF1mRNA) and vascular endothelial grow th factor (VEGF) during early fracture healing of rats with Huoxue Huayu de coction(HXHYD, ) . Methods:SpragueDawley rats( n = 72) were caused standardized closed left t ibial fracture under isofluraneanesthetize with a modified technique. 36 rats were treated w ith HXHYD after fracture. The other 36 rats wit hout medicine served as controls.Fractured sides w ere determined with H IF1 mRNA and VEGF expression on 1, 2, 3, 5,7 and 14 d.T hree normal rats wit hout any medicine served as nor mal controls. Results:The expression of HIF1mRNA was increased significantly on the fracture sides 2 days after fracture but decreased significantly after treatment w ith HXHYD on 1, 2 d( P 001) . In the same t ime,the expression of VEGF w as increased significantly after treatment by HXHYD on 3, 5, 7 and 14 d( P 005) .The expression of HIF1mRNA and VEGF w ere not detected in normal control group. Conclusion: T his study has confirmed that f