利用固定化金属亲和层析和质谱技术鉴定化脓性链球菌铜结合蛋白.PDF

微生物学通报 NOV 20, 2010, 37(11): 1656−1662 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 利用固定化金属亲和层析和质谱技术鉴定 化脓性链球菌铜结合蛋白 Δ Δ * 贺翔 银兴峰 张留辉 阳小燕 孙雪松 (暨南大学生命与健康工程研究院 广东 广州 510632) 摘 要: 化脓性链球菌是一种常见的高致病性革兰氏阳性菌, 每年都在全球范围内引发大量的感染 现象与病死案例。金属蛋白在微生物的代谢以及毒性构成中的作用已经逐渐得到重视。通过固定化 金属离子亲合层析技术对化脓性链球菌中铜结合蛋白进行分离, 配合质谱技术的使用, 共鉴定出 21 个铜结合蛋白, 这些蛋白分别在翻译、代谢、离子通道等各个生理进程中发挥相应作用。 关键词: 化脓性链球菌, IMAC, 金属蛋白, 铜结合蛋白 Identification of Copper Binding Proteins of Streptococcus pyogenes with IMAC and LC-MS/MS HE XiangΔ YIN Xing-FengΔ ZHANG Liu-Hui YANG Xiao-Yan SUN Xue-Song* (Institute of Life and Health Engineering , Jinan University, Guangzhou, Guangdong 510632, China) Abstract: Streptococcus pyogenes is a common pathogenic Gram-positive bacterium causing world- wide diseases with high morbidity and mortality every year. Metalloproteins have been highlighted for their essential roles in the metabolisms and virulence of pathogens. In this study, Cu-binding proteins of Streptococcus pyogenes were isolated by using immobilized metal ion chromatography and identified with LC-MS/MS. Totally, 21 proteins were identified and the network of interactions of the proteins was established. These proteins function in many biological processes such as translation, metabolism and ion transporting. Keywords: Streptococcus pyogenes, IMAC, Metalloproteins, Cu-binding proteins 金属蛋白在生物体中的重要性已经得到了越来 电子转移与基因转录过程的顺利进行, 以及氧的传 越多的重视。作为一类成员繁杂的蛋白质 , 金属