在体兔窦房结电生理功能中的缺血再灌注损伤研究.docVIP

　　在体兔窦房结电生理功能中的缺血再灌注损伤研究 --缺血再灌注对在体兔窦房结电生理功能的损伤研究 【摘 　要】目的　探讨缺血再灌注对在体兔窦房结电生理功能的影响。方法　取家兔90只随机分为对照组、缺血10、30、60、120 min组及缺血再灌注4 h组,每组10只。通过结扎及放松右冠状动脉起始部建立在体兔窦房结缺血再灌注损伤模型,同步记录体表及心腔内电图,观察AA间期变化及心律失常发生情况。结果　①对照组各时相点AA间期比较均无显著差异(P0.05)。②缺血组与缺血再灌注组80只兔子在缺血期共有51只(63.75%)发生窦性停搏、窦性心动过缓伴不齐、或房性快速性心律失常,且随缺血时间延长,心律失常发生率增加;有54只兔子(占67.5%)于结扎右冠状动脉后AA间期延长40 ms以上。③缺血再灌注组40只兔子中,有26只兔子于缺血期发生了窦性或房性心律失常,其中15只于再灌注后20 min内恢复正常,但另有3只发生了新的心律失常。④缺血再灌注组于缺血期有AA间期延长的27只兔子,多在再灌注后10 min内恢复至结扎前水平,但缺血60、120 min再灌注组则随再灌注时间进一步延长,AA间期再次延长。结论　①约2/3的兔子窦房结动脉可能起源于右冠状动脉。②窦房结缺血引起的心电生理变化与病窦综合征的心电图表现相似。③较长时间缺血后再灌注可发生再灌注心律失常。 【关 键 词】窦房结细胞|缺血再灌注|电生理|兔 Effect of ischemia reperfusion on electrophysiological function of sinoatrial node in rabbits 【Abstract】Objective　To study the effect of ischemia reperfusion(IR) on electrophysiological function of sino-atrial node(SAN) in rabbits.Methods　Niy healthy adult rabbitsly into control group, isch-emia groups (I) 10(I10 min), 30(I30 min), 60(I60 min) and 120 min(I120 min) and IRgroups (10, 30, 60 and120 min ischemia folloinR4 h, I30 minR4 h, I60 minR4 h and I120minR4 h). Thereodel ofSANiaber electrography synchronously.Results　①There e points in the control group.②Sinus arrest(SA), sinusbradycardia ia(SB), or atrial tachyarrhythmia(AT) ic period. The incidence rate of arrhythmia increased in a time-dependent manner.The AA intervals also increased by at least 40 ms in 54 rabbits (67.5%).③Sinus or atrial arrhythmia during isch-emic periodal after reperfusion.④IncreasedAA intervals ic period. Most of them recovered topre-occlusion level in after reperfusion, but the AA intervals prolonged again in I60 minR4 h and I120minR4 h groups as the reperfusion elongated.Conclusion　①These findings suggestthat about 2/3 of the rabbit si-nus node arteriesmaystemfromright coronary artery.②Electrophysiological changes due to ischemia ofSANresem-ble the electrocardiogram of sick sinoatrial node syndrome.③Reperfusion arrhythmia can be induced by reperfusionafter a long time of ischemia. 【Ke