氯喹对哮喘小鼠气道高反应性的抑制作用.PDFVIP

·12 · doi 10.3969/j.issn. 1673-4254.2015.01.03 J South Med Univ, 2015, 35(1): 12-16 基础研究 氯喹对哮喘小鼠气道高反应性的抑制作用氯喹对哮喘小鼠气道高反应性的抑制作用 孙晓春，胡晓燕，王莉佳，刘恩梅，符 州 重庆医科大学附属儿童医院呼吸病学实验室，重庆 400014 摘要：目的探讨氯喹对哮喘小鼠气道高反应性的作用及其可能机制。方法 Balb/c 小鼠随机分为对照组、哮喘组、氯喹治疗组、 地塞米松治疗组、氯喹+地塞米松治疗组，除对照组外，其余各组小鼠均给予OVA 致敏和激发，建立哮喘小鼠模型，于每次激发 前腹腔注射给药，对照组则于同一时间予等量PBS 处理，末次激发后24 h 内小鼠肺功能仪检测小鼠气道高反应性；检测支气管 肺泡灌洗液（BALF）中细胞总数及分类计数；制作肺组织病理切片，HE 染色后光镜下观察病理变化；ELISA 检测支气管肺泡灌 洗液中细胞因子IL-6、PGF2α水平。结果 氯喹可明显降低哮喘小鼠气道高反应性（吸入25 mg/ml Ach 时4.5±0.4 vs 6.1±0.9 ，P 0.05 ，吸入50 mg/ml Ach 时4.6±0.5 vs 8.2± 1.0 ，P0.001）；明显降低哮喘小鼠支气管肺泡灌洗液中炎症细胞数（P0.01）；显著降 低哮喘小鼠病理评分（P0.05）；可一定程度降低哮喘小鼠BALF 中PGF2α因子水平；与地塞米松合用可明显减轻哮喘小鼠支气 管细支气管周围炎症（P0.05）、血管周围炎症（P0.01）、肺组织病理评分（P0.001），明显降低哮喘小鼠BALF 中IL-6 因子水平 （P0.05）。结论 氯喹可抑制哮喘小鼠气道高反应性；地塞米松与氯喹合用较单独用药效果更好，可能对中性粒细胞参与的哮 喘有效。 关键词：哮喘；氯喹；气道高反应性；IL-6 ；PGF2α Inhibitory effect of chloroquine on airway hyperresponsiveness in asthmatic mice SUN Xiaochun, HU Xiaoyan, WANG Lijia, LIU Enmei, FU Zhou Department of Respiratory Medicine, Childrens Hospital Affiliated to Chongqing Medical University, Chongqing 400014, China Abstract: Objective To investigate the effect of chloroquine on airway hyperresponsiveness in asthmatic mice and explore the possible mechanism. Methods Balb/c mouse models of asthma established using OVA received intraperitoneal injections of chloroquine, dexamethasone, or both prior to OVA challenge. Within 24 h after the final challenge, airway hyper- responsiveness (AHR) of the mice was assessed, and the total cell count and the counts of different cell populations in the bronchoalveolar lavage fluid (BALF) were determined under light microscopy. The severity of lung inflammation was evaluated using HE staining, and the concentrations of IL-6 and PGF2α in the BALF were detected by enzyme-linked immunosorbent assay (ELISA). Results Chloroquine pretreatment