紫外吸收光谱原理（Principle of ultraviolet absorption spectrum）.docVIP

紫外吸收光谱原理（Principle of ultraviolet absorption spectrum） The basic principle of ultraviolet visible absorption spectrometer 2011-12-14 China Chemical Network Click: 484 by UV - visible absorption spectra for quantitative analysis of long-standing, can be traced back to ancient times, in the year 60 ancient Greece already know to estimate the content of iron in Schisandra extract by using vinegar, this ancient method was originally used to because of human detection, so called colorimetric method. The 16 and seventeenth Century, the correlation theory began to flourish, in 1852, Bill (Beer) reference Bouguer Lambert (Bouguer) and 1729 (Lambert) published in the 1760 article, put forward the basic law of spectrophotometry, the liquid layer with the same thickness, color intensity and color solution concentration the proportion, which lays a theoretical foundation by spectrophotometry, this is the famous Bill Lamberts law. Instrument formation Spectrophotometry is called spectrophotometric method, but the concept of spectrophotometry spectrophotometry is somewhat vague, refers to the function of the instrument, the instrument of light were determined by spectrophotometric method, this kind of instruments including spectrophotometer and atomic absorption spectrometer (AAS). The essence of absorption is the absorption of light, so it is reasonable to say that spectrophotometry is the most accurate. The ultraviolet visible absorption spectrum is produced by the absorption of light in the 200-800nm spectrum of the molecules in the material. This molecular absorption spectrum arises from the transition of electrons in valence electrons and molecular orbitals (electrons in an atom or molecule) in a state of motion. Each state has a certain energy, belonging to a certain energy level. These electrons, due to various causes (such as by excitation of light, heat, and electricity), move from one energy level to another, called transition. When these electrons absorb the energy of