ERF转录因子对生物胁迫的反应及对棉花抗性改良的意义.pdfVIP

分子植物育种，2008年，第6卷，第1期，第ll卜ll6页 MolecularPlantBreeding，2008，Vo1．6，No．1，ll卜ll6 专题介绍 Review ERF转录因子对生物胁迫的反应及对棉花抗性改良的意义 孟宪鹏 李付广‘刘传亮 张朝军 武芝侠 中国农业科学院棉花研究所，农业部棉花遗传改良重点开放实验室，安阳，455004 ’通讯作者．Lifg@cricaas．corn．cn 摘 要 大量研究表明植物中ERF(ethylene responsive factor)类转录因子广泛参与外界环境胁迫应答基因 表达的调控。它编码的蛋白能够特异结合GCC—box元件，从而调控启动子含有GCC—box的病程相关蛋白基 因的表达，在植物抗病反应中发挥重要的调控作用。棉花中ERF基因表达受生物和非生物胁迫诱导，并在乙 烯、茉莉酸和水杨酸信号传导途径中发挥一定的作用。一些ERF基因在转基因植物中的超表达表现出了一 定的广谱抗性，因而在棉花分子育种中具有较为广阔的应用前景。本文主要论述了棉花等植物中ERF的结 构与功能特征，当前相关研究进展，及其对棉花抗病性分子遗传改良的意义。 关键词 ERF(乙烯应答因子)，转录调控，棉花抗病性，分子改良 Responses ofERF Transcription Factors to Biotic Stresses and its Applications in Improving Cotton Resistance Meng Xianpeng Li Fuguang’Liu Chuanliang Zhang Chaojun Wu Zhixia Co,on Research Institute，Chinese Academy of Agricultural Sciences，Key Laboratory of Cotton Genetic Improvement，Ministry of Agriculture， Anyang，455004 ’Corresponding author，Lifg@cricaas．corn．cn Abstract Numerous studies have shown that transcription factor ERF(ethylene responsive factor)plays a crucial role in regulating plant genes that response to environmental stress．ERF is a subfamily of AP2／ERF，which could specifically bind the GCC—box presenting in the promoter ofmany PR genes and modulate their expression，conse- quently plays an important role in disease resistance signal pathway．Cotton ERF genes ale induced by biotic and abiotic stresses，and involved in ethylene，jasinine and salicylic signal pathway．Over—expression of ERF genes showed the broad—spectrum pathogen resistance in transgenic plants，which suggests a potential application of ERF genes in cotton molecular breeding．This paper summarized the structure and function of the ERF transcription factors in cotton and other plants，and discussed the significance in enhancing cotton disease resistance and molec— ular genetic improvement based on the current research progress． Keywords ERF(ethylene responsive factor)，Transcriptional regulation,Cotton disease resistance，Molecular im—