复合培养对低氧时肺动脉平滑肌细胞表达细胞因子的调节.docVIP

复合培养对低氧时肺动脉平滑肌细胞表达细胞因子的调节 张志远1，钱频2，王建春1，程序3，刘婷1，钱桂生1，王关嵩1（1第三军医大学新桥医院呼吸疾病研究所，重庆 400037； 2第三军医大学新桥医院超声科，重庆 400037；3重庆市垫江县中医院，重庆市垫江县 408343,） 提要 目的 研究低氧对肺动脉平滑肌细胞（PASMCs）白细胞介素-1β、4、10、13（IL-1β,4,10,13）活性的影响，及复合培养下肺微血管内皮细胞（PMVECs）对其调控作用。方法 大鼠PASMCs单独培养或与PMVECs复合培养，分为正常组、低氧作用2h（H2）、6h（H6）、12h（H12）、24h(H24)组，采用ELISA法测定各组培养细胞上清中IL-1β、IL-4、IL-10、IL-13的活性。 结果 低氧刺激后PASMCs的IL-1β、IL-4、IL-10、IL-13活性2h开始升高，6h达高峰，12h降低；各时相点复合培养组均低于对应的单独培养组（P0.01）。结论 低氧可以激活PASMCs的IL-1β、IL-4、IL-10、IL-13活性，在复合培养条件下，PMVECs对此效应具有下调性影响。 关键词 低氧；复合培养；肺动脉；平滑肌细胞；肺微血管内皮细胞;白细胞介素 The regulation of expression of cytokines in hypoxia of pulmonary arterial smooth muscle cells by co-culture ZHANG Zhi-yuan, QIAN Pin, WANG Jian-chun, CHENG Xu, LIU Ting, QIAN Gui-sheng , WANG Guan-song ( Institute of Respiratory Diseases , Xinqiao Hospital , Third Military Medical University , Chongqing 400037 , China) Abstract : Objective 　 To study the effect of hypoxia on the expression of interleukin1β,4,10,13 (IL-1β,4,10,13) in pulmonary arterial smooth muscle cells (PASMCs) and the regulation role of cocultured pulmonary microvascular endothelial cells(PMVECs). Methods Rat PASMCs were cocultured with or without rat PMVECs, and randomly divided into 5 groups : normal group (N) , 2 h hypoxia (H2) , 6 h hypoxia (H6) ,12 h hypoxia (H12) , and 24 h hypoxia (H24) . The expression level of IL－1β、IL－4、IL－10、IL－13 in the supernatant was detected with ELISA. Results 　The expression level of IL－1β、IL－4、IL－10、IL－13 increased in H2 , reached the highest in H6 , and decreased in H12. The expression of cocultured group was lower than in homotype group. Conclusion Hypoxia can enhance activities of IL-1β, IL-4, IL-10, and IL-13 in PASMCs , which can be down-regulated by cocultured PMVECs . 　　Key words : hypoxia ; coculture ; pulmonary artery ; smooth muscle cells ; pulmonary microvascular endothelial cells; interleukin 低氧性肺血管重构是原发性肺动脉高压、肺心病等多种呼吸系统疾病的共同的病理基础。在体情况下，是由内皮细胞、平滑肌细胞等多种细胞与细胞外基质共同参与的复杂的病变过程，多种细胞相互调控、相互影响、以一种网络的形式发挥作用。既往的研究多集中于低氧对PA