复烤片烟醇化过程中几种化合物含量及相关酶活性的变化.PDFVIP

78 中国烟草科学 2010 ，31 （3 ）：78-81 复烤片烟醇化过程中几种化合物含量及相关酶活性的变化 1 1* 2 3 4 5 武德传 ，周冀衡 ，李章海 ，徐晓燕 ，周初跃 ，张西仲 (1.湖南农业大学烟草工程技术研究中心，长沙 410128 ；2. 中国科学技术大学烟草与健康研究中心，合肥 230051 ；3.中国科学 技术大学地球与空间科学学院，合肥 230026 ；4.安徽中烟工业公司，合肥 230088 ；5.贵州省烟草公司黔南州公司，贵州 都匀 558000) 摘 要：对醇化时期不同阶段复烤片烟的总多酚(绿原酸和芸香苷之和) 、总脂质、淀粉和蛋白质的含量及多酚氧化酶 (polyphenol oxidase, PPO)、脂氧合酶、α-淀粉酶和蛋白酶活性进行了测定。结果表明，经过18 个月的醇化，多酚、总脂质、 淀粉和蛋白质的含量分别下降20.0%, 15.0%, 11.5%和12.2%。醇化前后，芸香苷、淀粉和蛋白质含量差异不显著，总脂质含 量差异达显著水平，总多酚、绿原酸含量差异达极显著水平。PPO 、脂氧合酶、α-淀粉酶和蛋白酶的活性呈下降趋势，醇化 前后4 种酶活性差异均达极显著水平。 关键词：复烤片烟；烤烟；醇化；酶；化合物 中图分类号：TS413 文章编号：1007-5119 （2010 ）03-0078-04 DOI: 10.3969/j.issn.1007-5119.2010.03.018 Changes in Chemical Compounds and Enzyme Activity in Redried Lamina of Flue-cured Tobacco during Aging 1 1* 2 3 4 5 WU Dechuan , ZHOU Jiheng , LI Zhanghai , XU Xiaoyan , ZHOU Chuyue , ZHANG Xizhong (1. Research Center of Tobacco Engineering and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Center of Tobacco and Health Research, University of Science and Technology, Hefei 230051, China; 3. Department of Earth and Space Science, University of Science and Technology, Hefei 230026, China; 4. China Tobacco Anhui Industrial Corporation, Hefei 230088, China; 5. Tobacco Corporation of Qiannan Autonomous Prefecture, Duyun, Guizhou 558000, China) Abstract: Contents of polyphenol (chlorogenic acid and rutin), lipid, starch and protein, and activities of relevant enzymes, including PPO, lipoxygenase, α-amylase, and proteinase, in redried lamina of flue-cured tobacco were examined du