人剪切修复基因XPD对p52及p21基因影响.pdfVIP

摘要 摘 要 目的：探讨人剪切修复基因XPD转染／＼HepG2肝癌细胞后p52矛lp21基因表达的变 化以及对肝癌细胞生长的影响。 2000H旨质体转染 方法：人肝癌细胞HepG2为靶细胞，XPD基因通过Lipofectamine 和蛋白印迹(Western 白质的表达量，并用四甲基偶氮哗盐微量酶反应比色(MTT)法检测各组细胞 的增殖能力。 达较其他2组显著升高(均P0．01)。Western blot法检测显示在XPD组中p52的蛋 白表达较N2组和空白对照组显著下降，而基因XPD及p21的蛋白表达较N2组和空 HepG2后细胞增殖能力减弱。 结论：XPD基因可以抑制癌细胞的生长和基因p52的表达，促进p21的表达。 Abstract ABSTRACT the effectsofthetransfectedXPDon Objective：Toinvestigate biological gene cell hepatomaHepG2． Methods：TheXPD wastransfectedinto cell with gene hepatomaHepG2 2000．Therewerethree in this Lipofectamine groups studyincluding HepG2一pEGFP—N2-XPDgroup(XPDgroup)、HepG2一pEGFP-N2group(N2group) of and 1 detected andblankcontrol were group．TheexpressionsXPD，p52p2 by andWesternblot．Thecell WasexaminedMTT． RT-PCR cycle by withblankcontrol andN2 levelof Results：Compared group group，theexpression in mRNAdeclined XPD the levels p52 significantlygroup(P0．01)；butexpressions ofXPDand1 mRNAwereelevated inXPD trends p2 obviouslygroup(PO．O1)．The of and1 detectedWesternblotwereconsistentwithtrendof XPD，p52p2protein by thatcell inXPD mRNA．MTTresultsshowed increased