大鼠虚寒证模型建立、评价及附子对虚寒证大鼠影响的研究.pdfVIP

Study on the Establishment and Evaluation of Eeficiency-cold Model in Rats and the Influence of Mankshood on this Model Speciality: Research on microcirculation of brain and tumor in the field of integrated TCM and Western Medicine Tutor: Prof. Wang Shijun Author:Zhao Jian Abstract Objective: To explore methods of establishment and evaluation of deficiency-cold model in Rats, and to abserve the effect of monkshood, with heat properity acroding to the theory of TCM, on energy metabolism and on relevant indicators in deficiency-cold model in rats. To analyse the tendency and speciality of these effects, then find the signs of cold and heat, and reveal the inherent relationship to cold and heat properity of TCM. To provide a useful guidance for clinical medicine. Methods: In order to induce deficiency-cold model in rats, decoction of four Chinese medicinal herbs with cold property, gypsum, radix gentianae, golden cypress and rhizome anemarrhenae decoction were administered intragastrically to rats for 14 days. After the model was successfully produced, decoction of mankshood was given for 7 days for treatment. The effects on temperature and cold and heat preference in deficiency-cold model rats after drug administration were observed. Collecting the food, water, urine and dung in quantity of rats for 24 hours with metabolic cage .The EI, DE, FE and ME were detected by Oxygen Heat Calculation. T3, T4, TSH, TRH in blood serum were analyzed using Enzyme-linked immunosorbent assay. Fasting blood sugar, hepatic glycogen and LD levels were measured. The activities of hepatic ATPase, LDH and SDH in liver were analyzed using measuring kit respectively. The EC and the content of ATP in liver were dected using HPLC. Results: R