增生性瘢痕和瘢痕疙瘩组织中TGF-β1及Ⅰ.doc

增生性瘢痕和瘢痕疙瘩组织中TGF-β1及Ⅰ、Ⅲ型胶原基因表达的改变及意义 文章来源： 　　　　 2006-7-26 17:12:41 ? 增生性瘢痕和瘢痕疙瘩组织中TGF-β1及Ⅰ、Ⅲ型胶原基因表达的改变及意义 中华整形烧伤外科杂志 1999年第4期第15卷 论著 作者：唐世杰　庞素芳　曹亚　李晓艳　王磊 单位：唐世杰　汕头大学医学院附属第二医院烧伤整形科　515041；庞素芳　曹亚　李晓艳　王磊　湖南医科大学 　　【摘要】　目的　检测增生性瘢痕(H)和瘢痕疙瘩(K)组织中TGF-β1及Ⅰ、Ⅲ型前胶原mRNA的表达，了解其相互关系及意义。方法　斑点杂交分析检测H和K组织中Ⅰ、Ⅲ前胶原及TGF-β1 mRNA稳态水平的改变；原位杂交检测TGF-β1 mRNA在组织中的空间分布。结果　①K和H组织中TGF-β1 mRNA稳态水平明显高于N组和S组；②K选择性Ⅰ型前胶原mRNA表达增强，而H组织中Ⅰ、Ⅲ前胶原mRNA表达均增强。结论　TGF-β1在H和K的发病机理中起了重要作用，K和H发生发展中具有不同的分子机理。 Changes in TGF-β1 and type Ⅰ，Ⅲ procollagen gene expression in keloid and hypertrophic scar TANG Shijie*, PANG Sufang, CAO Ya,et al.The Department of Burn and Plastic Surgery,The Second affiliated Hospital of Shantou University Medical College, Shantou 515041 　　【Abstract】　Objective　The expression of mRNA for TGF-β1,typeⅠ,Ⅲ procollagen in keloid and hypertrophic scar was investigated in order to elucidate the pathogenesis of and the difference between keloid and hypertrophic scar.Method　Dot-blot hybridization analysis was used to examine the type Ⅰ,Ⅲ procollagen and the expression levels of steady-state mRNA.In situ hybridization allowed direct assessment of the distribution of TGF-β1 mRNA in the tissue.Results　①Our study indicated that the expression level of mRNA for TGF-β1 significantly increased in keloid and hypertrophic scar compared with normal scar and normal skin.②In keloid tissues,type Ⅰ precollagen mRNA expression was selectively increased.However,in hypertrophic scar,type Ⅰ and Ⅲ collagen mRNAs expressions were simultaneously increased.Conclusion　TGF-β1 plays an important role in the pathogenesis of keloid and hypertrophic scarring.It implicates that distinct molecular mechanisms are operative in the development of keloid and hypertrophic scarring. 　　【Key words】　Hypertrophic scar　　Keloid　　mRNA expression　　Collagen　　TGF-β1 　　增生性瘢痕(H)和瘢痕疙瘩(K)是皮肤损伤后主要以胶原蛋白沉积为特征的病理性真皮纤维化，两者具有不同的临床表现和生物学行为，对其发病机理尚不完全清楚。有研究表明：在一些纤维化疾病中不仅显示出过量的细胞外基质的堆积，而且还有TGF-β1的增高［1］，但是TGF-β1在瘢痕组织中的表达研究不多。为此，我们采集H和K的活体组织标本，运用分子杂交技术，对T