人类巨细胞病毒对粒系祖细胞分化过程中HOXA9基因表达的影响.docVIP

题目： 人类巨细胞病毒对粒系祖细胞分化过程中zuoma78＠163.com 　　　 －lwjlyfy@ 基金项目 ： 四川省教育厅重点科研基金(2004A058) 人类巨细胞病毒对粒系祖细胞分化过程中Hematopoietic Stem-Progenitor Cell，HSPCHuman Cytomegalovirus ,HCMV）进行干扰，探讨HCMV对HOX A9基因的影响。方法 体外培养，滴度为106蚀斑形成单位plaque formation unit) PFU/ml，按0.1ml 109 PFU /ml接种培养体系。采用测定表达。结果（P0．0）Hox A9基因 Expression of HOXA9 homeobox gene in Granulocyte differentiation infected with HCMV Chenai, Chenhongying , Chenjunhong , Liu wenjun Department of pediatrics, affiliated hospital of LuZhou medical college, LuZhou 646000, China [Abstract] Objective To investigate the expression of HOXA9 gene on the differentiation of hematopoietic stem-progenitor cell to colony forming unit-granulocyte of normal group and HCMV group. Methods Normal CFU–G culture was used as blank control . After MTT detection , HCMV of 106 PFU(plaque formation unit, PFU) /ml was diluted to 0.1ml 109 PFU /ml and added into the infected group. The expression of HOX A9 gene by Real-time Quantitative Polymerase chain Reaction method. Results 1. Compared with the expression on day 3 , the level of HOX A9 was obviously higher on day 7 and lower on day 12 respectively in each group（ P＜0. 05）Compared with the normal group, the expression of HOXA9 of were down-regulated by HCMV (p0.05).Conclusions ①Homeobox genes (HOX) have a regulatory function in the differentiation process of hematopoiesis. The HOXA9 gene significantly play a important role in the process of Granulocyte differentiation. ② HCMV (0.1ml 109 PFU /ml) can down-regulate the expression of HOXA9 and lead a suppression effect on the cell morphology , which confirm the theory that the normal hematopoietic lineage determination and maturation rely on the stable and consistently expression of Homeobox genes. Key Words: Human Cytomegalovirus; Colony Forming Unit-Granulocyte; HOXA9 homeobox gene. 基金项目：四川省教育厅重点科研基金(2004A058) 作者单位：646000四川泸州，泸州医学院附属医院儿科 人类巨细胞病毒（Human cytomegalovirus, HCMV）Hematopoietic Stem-Progenitor Cell，HSPCHCMV对脐血造血祖细胞抑制作用的机制具有