心肌声学造影评价大鼠心脏移植急性排斥反应模型心肌血流灌注的动态.docVIP

心肌声学造影评价大鼠心脏移植急性排斥反应模型心肌血流灌注的动态 华 兴1，吴 蔚2，卓丽莎3，丁 俊1，何 芸1，陈朝辉1（400038重庆，第三军医大学西南医院：超声科1，胸心外科2；400037重庆，第三军医大学新桥医院超声科3） [摘要]目的 评价心肌声学造影在检测大鼠心脏移植急性排斥反应心肌灌注变化中的价值。方法 建立大鼠心脏移植模型（同种异体移植18只，同系移植12只），按移植后第2、4、6天分为3组（每组同种异体移植6只、同系移植4只），分别行心肌声学造影，自动拟合曲线，比较不同组间A、β及A×β值差异及同组内同系移植与同种异体移植大鼠间差异。结果 同种异体移植大鼠于术后第6天A、β（P＜0.05）、A×β值（P＜0.01）显著降低，同系移植大鼠术后第2天低于第4、6天（P＜0.05）。在同种异体与同系比较中，A与β值于术后第6天均有差异（P＜0.05），而A×β值则于术后第4天出现差异（P＜0.05），到第6天差异更加显著（P＜0.01）。结论 心肌声学造影可反映大鼠心脏移植后心肌灌注的变化规律，对于急性排斥反应的诊断与监测具有应用价值。 [关键词]心肌声学造影；心脏移植；急性排斥反应；心肌灌注 Dynamic Evaluation to Myocardial Perfusion in Acute Rejection Model of Rat Heart Transplantation with Myocardial Contrast Echocardiography Hua Xing1，Wu Wei2，Zhuo Lisha3，Ding Jun1，He Yun1，Chen Zhaohui1（1Dept. Ultrasound of Southwest Hospital affilliated to the Third Military Medical University 2. Dept. Cardio-Thoracic Surgery of Southwst Hospital affilliated to the Third Military Medical University 3. Dept. Ultrasound of Xinqiao Hospital affilliated to the Third Military Medical University） [Abstact]Objective To evaluate the value of myocardial contrast echocardiography （MCE） in the detection of acute rejection of heart trasnplantation in rats. Methods Models of heart transplantation in rats were constructed （18 allografts and 12 isografts）. The rats were devided into 3 groups according to the 2nd， 4th and 6th day post transplantation （6 allografts and 4 isografts in each）. MCE was performed in each rat and automatic fitting curve was achieved. The values of A， β and A×β were compared between the 3 groups and between allo- and iso-graft in each group. Results The values of A and β were decreased at the 6th d post trasplantation in allograft （P＜0.05）， and A×β was also decreased at the 6th d in allograft （P＜0.01） while lower at the 2nd d than that in the 4th d and 6th d in isograft（P＜0.05）. Compared between the allograft and isograft， there was a difference in A and β at the 6th d （P＜0.05） and A×β at 4th d （P＜0.05）and 6th d（P＜0.01）. Conclusion MCE can reflect th