电极位置对蟾蜍坐骨神经干动作电位波形记录的影响.docVIP

电极位置对蟾蜍坐骨神经干动作电位波形记录的影响 张开元1，王冠忠1，王付凡1，廖盛涛1，王艺杰1，杨丽2，张骏2 （400038 [摘要] 目的 研究引导电极之间、引导电极与刺激电极之间的距离对蟾蜍坐骨神经干动作电位波形记录的影响，阐明“两相波形不对称”这一现象蕴含的生理机制。方法 制备坐骨神经干标本，采取麻醉阻滞单相动作电位记录法、双相动作电位记录法、单/双相动作电位记录对比法，观察刺激电极间位置以及引导电极与刺激电极间位置对神经干复合动作电位的波形、幅度和时程的影响。结果 刺激电极与引导电极间距离对单相动作电位波形的影响：通过单相动作电位记录，当刺激电极与引导电极间距离在3.0-5.5 cm范围内逐渐增大时，波幅呈降低趋势，时程呈增大趋势。引导电极间距离对双相动作电位波形的影响：通过双相动作电位记录，当两个引导电极间距离在0.1-2.0 cm范围内逐渐增大时，负相、正相波波幅均显著升高，两电极间距 2 cm范围内逐渐增大时，负相波波幅与时程相对稳定，而正相波波幅逐渐减小而时程逐渐增大。通过单/双相动作电位对比记录同一位置的动作电位波形进行比较：当两引导电极间距 2.0 cm时，两种方法记录到的动作电位幅度有显著差别（P 0.05）且单相动作电位幅度显著大于双相动作电位；当两引导电极间距 2 cm时，两种方法记录到的动作电位幅度和时程没有明显差别（P 0.05）。结论 当两引导电极间距较小时，影响动作电位波形的主要因素是两引导电极之间的距离大小决定的“周期效应”，即间距在兴奋区域波波长内的两个引导电极引导出的电位差较兴奋区域波长外的两个引导电极间的电位差有明显差别。当两引导电极间距较大时，影响动作电位波形的主要因素为引导电极与刺激电极之间的距离大小决定的“迁延效应”，即传播过程中，众多神经纤维动作电位叠加形成的神经干复合动作电位的幅度逐渐变小、波长逐渐变大，且这种影响在传播距离较长时才明显。 [关键词] 神经干；动作电位；刺激电极；引导电极 The effect of the electrode spacing on the toad sciatic nerve-trunk action potential waveform’s recording Zhang Kai-yuan1, Wang Guan-zhong1, Wang Fu-fan1, Liao Sheng-tao1, Wang Yi-jie1, Yang Li2, Zhang Jun2 (1Cadet Brigade, 2Departments of Physiology, Third Military Medical University, Chongqing, 400038, China) [Abstract] Objective To explore the effect of the electrode placing between stimulating (S) and guiding (R) electrodes as well as that between the R electrodes on the sciatic nerve action potential waveform’s recording, and clarify the mechanism of asymmetric two-phase waveform. Methods We prepared sciatic nerve-trunk specimens, use anesthetic block monophasic action potential, biphasic action potential and the contrastive mono / biphasic action potential recording method to observe the waveform, amplitude, and duration of nerve-trunk action potential waveform. Results In monophasic recording, the amplitude decreases and the duration increases with the spacing between S and R- increasing from 3.0 cm to 5.5 cm. In biphasic recordings, the amplitude of both negative and positive negative phase waves have a tendency to improve w