谷物及蔬菜等农作物中乙草胺丁草胺残留的测定方法研究.docVIP

 本科毕业设计说明书（论文） （2010届） 题目：谷物及蔬菜等农作物中乙草胺、丁草胺残留的测定方法研究 学生姓名 岑梅娜 指导教师 刘文涵 陈小珍 专业班级 应用化学 2006级（3）班 所在学院 化学工程与材料科学学院 提交日期 2010年6月 谷物及蔬菜等农作物中乙草胺、丁草胺残留 的测定方法研究 摘 要 改进国标GB/T5009.164－2003《大米中丁草胺残留量的测定》的测定方法，建立了气相色谱和气相色谱-质谱方法测定谷物及蔬菜等农作物中乙草胺、丁草胺残留量的方法, 试样中乙草胺、丁草胺残留用正已烷-丙酮提取,再用过弗罗里硅土固相萃取柱净化, 用气相色谱-质谱联用仪测定, 分析方法满足残留分析的要求。采用添加法测定回收率, 添加水平为0.01mg/kg～0.5mg/kg 时, 大米中乙草胺回收率在85.3%～90.6%，RSD在2.23%～5.05%之间，丁草胺回收率在85.2%～95.6%，RSD在2.02%～4.58%之间；黄瓜、西瓜等蔬菜水果中乙草胺回收率在81.0%～96.4%，RSD在2.82%～5.26%之间，丁草胺回收率在82.2%～92.6%，RSD在2.10%～4.61%之间。 本方法大米的检出限为0.002mg/kg，本方法蔬菜、水果中的检出限为0.005mg/kg本方法灵敏度高, 速度快, 重复性好。 关键词：气相色谱-质谱联用仪；乙草胺；丁草胺；残留量  ABSTRACT In order to improve the national standard GB/5009.164-2003-“the determination of butachlor in rice”, a rapid method has been developed for the determination of acetochor and butachlor residues in grain and vegetable by gas chromatography and gas chromatography-mass spectrometry. Acetochor and butachlor in samples are extracted with hexane – acetone, and then cleaned up by Florisil solid-phase extraction. The acetochor and butachlor residues are detected by gas chromatography-mass spectrometry. And analysis methods meets the residual analysis requirements. Determined the recovery rate by adding the standard solution and the linear range is from 0.01mg/L to 0.5mg/L. The recoveries of Acetochor in rice ranged from 85.3 % to 90.6% with the relative standard deviations (RSD) ranged from 2.23% to 5.05%, while butachlor’s recoveries ranged from 85.2% to 95.6% with the relative standard deviations (RSD) ranged from 2.02% to 4.58%. And the recoveries of Acetochor in fruits and vegetable, such as Cucumber and watermelon, ranged from 81.0% to 96.4% with the relative standard deviations (RSD) ranged from 2.82% to 5.26%, while butachlor’s recoveries ranged from 82.2% to 92.6% with the relative standard deviations (RSD) ranged from 2.10% to 4.61%. The limited of detection (LOD) is 0.002mg/L in ri