warthin-starry银染色方法显示空气细颗粒物pm国家科技图书文献.doc

Warthin-Starry银染色方法显示空气细颗粒物（PM2.5）的探讨 白玉萍 刘红刚 徐东群 岳常丽 王秦 黄宁华 [摘要] 目的 探讨光镜下显示组织或细胞内空气细颗粒物（PM2.5）的方法，为PM2.5和人体健康与疾病关系的研究提供手段。方法 对HE染色、Warthin-Starry银染色方法(WS银染色)显示游离PM2.5及肺组织中尘粒的染色效果进行对比，用免疫组织化学En Vision法标记吞噬细胞、尘细胞，用透射电镜（TEM）观察两种颗粒的超微结构，用X-线质谱元素分析仪（X-RSA）等分析两种颗粒的元素成分。结果 WS染色方法可以显示游离的PM2.5及吞噬细胞内的尘粒，且可显示HE染色下不被显示的微细尘粒；两种尘粒的超微结构相近，主要构成元素也相同，均为硅（Si）。结论 肺尘细胞内的尘粒是游离PM2.5被吞噬细胞吞噬处理后的一种细胞内形式；WS银染色法可以较好地显示不同状态的PM2.5，可做为光镜下观察PM2.5和健康与疾病关系的一种常规染色方法。 [关键词] 空气细颗粒物；尘粒；染色与标记 Investigation of the method for showing fine atmospheric particulate matter (PM2.5) with Warhtin-Starry silver staining BAI Yu-ping, LIU Hong-gang*, XU Dong-qun, YUE Cang-li, WANG Qin, HUANG Ning-hua. *Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.( BAI Yu-ping, LIU Hong-gang, YUE Cang-li), Institute for Environmental Health and Related Products Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. (XU Dong-qun, WANG Qin, HUANG Ning-hua) Corresponding author: LIU Hong-gang (liuhg1125@163.com), XU Dong-qun (dongqunxu@) [Abstract] Objective To explore the method for showing fine particulate matter (PM2.5) in tissues or cells so as to offer an approach for researching of the relationship between PM2.5 and human health status. Methods Staining effects of hematoxylin-eosin staining method (HE staining) and Warhtin-Starry silver staining method (WS staining) on PM2.5 in Beijing area and dust granules in pulmonary dust cells of autopsy samples were compared. Phagocytes and the dust cells were stained with immunohistochemical En Vision method. The ultrastructure and chemical component of PM2.5 and dust granules were observed and analyzed by transmission electron microscope (TEM) and X-Ray spectrum analysis（X-RSA） respectively. Results Both HE staining and WS staining could show PM2.5 and dust granules. Moreover, WS staining could also show smaller or minute dust granules which could not be shown by HE staining. The ultrastruc