不同浓度的uspio和goldmag-cs磁共振成像信号-第三军医大学学报.doc

USPIO和GoldMag磁共振成像信号特点的实验研究 逄 鑫，邹利光，杨 华，张 辉，舒通胜，张 松（第三军医大学新桥医院放射科，重庆 400037） 摘 要：目的 通过对不同浓度超小超顺磁性氧化铁（ultra-small superparamagnetic iron oxide，USPIO）和金磁微粒（GoldMag-Coreshell, GoldMag）行不同序列MR成像，对比分析其信号特点，探讨合适的MRI检查方法。方法 以磷酸盐缓冲液（PBS）为稀释剂将USPIO和GoldMag分别稀释为0.5～1000 μg/ml的47个不同的浓度，以PBS液为对照，共获得48个浓度,行FSE T1WI、FSE T2WI和GRE T2*WI磁共振成像。观察不同浓度USPIO和GoldMag在3种序列中的信号强度变化规律，计算信号强度变化比率，绘制浓度-信号强度曲线图。结果 GoldMag与USPIO的MRI信号变化规律基本一致，随浓度增加，FSE T1WI信号强度呈升高趋势，FSE T2WI信号强度略有下降，GRE T2*WI信号强度明显降低。不同成像序列两种对比剂信号强度比较，在FSE T1WI，浓度高于30 μg/ml时信号强度有显著性差异（P 0.05）；在FSE T2WI，浓度为40～90 μg/ml时信号强度有显著性差异 （P 0.05）；在GRE T2*WI，两种对比剂信号强度无显著性差异 （P 0.05）。结论 GoldMag与USPIO常规MRI信号特点变化趋势相似，GoldMag是较理想的MRI分子影像学研究纳米粒对比剂，GRE T2*WI为USPIO和GoldMag的最佳成像序列。 关键词：超小超顺磁性氧化铁；金磁微粒；磁共振成像；信号强度 中图法分类号： 文献标识码：A Experimental study of MRI signal characteristics of USPIO and GoldMag PANG Xin, ZOU Li-guang, YANG Hua, ZHANG Hui, SHU Tong-sheng, ZHANG Song (Department of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China) Abstract: Objective To investigate the MRI signal characteristics of Ultra-small Superparamagnetic Iron Oxide (USPIO) and GoldMag-Coreshell (GoldMag) at different concentration and get the appropriate MR imaging method. Methods The experiment was performed with USPIO and GoldMag, including 47 samples of different concentration of contrast medium. The samples of each group were diluted with PBS, and the concentration was from 0.5μg/ml to 1000μg/ml. One milliliter of different concentration contrast medium was filled in EP tube and examined by FSE T1WI, FSE T2WI, and GRE T2*WI. Results The MRI signal intensity of USPIO and GoldMag varied consistently. Along with the concentration increase, the signal intensity increased at FSE T1WI, descended a little at FSE T2WI, and dropped down obviously at GRE T2*WI. The signal intensity of the two contrast media under three different sequences was also compared. At FSE T1WI, the signal intensity of two contrast media （＞30μg/ml） wa