实时荧光定量逆转录聚合酶链式反应联合检测食管-第三军医大学学报.docVIP

实时定量PCR检测食管癌患者组织和外周血中TACSTD1和CK19表达的临床意义 张 浩，陈家军，聂荣华，洪 波，刘日辉，张拥军，詹波涛（441021襄樊，襄樊市中心医院心胸外科） [摘要] 目的 研究食管癌患者外周血中TACSTD1和CK19的表达，探讨其作为食管癌微转移标志物的可行性和其对食管癌患者预后的影响。方法 用实时荧光定量RT-PCR联合检测30例食管癌患者手术切除的肿瘤标本和食管残端TACSTD1和CK19的表达并比较二者的区别，检测30例健康血员外周血标本TACSTD1和CK19的表达得出正常人群的表达范围，以此为标准检测30例食管癌患者术前外周血标本是否存在TACSTD1和或CK19的高表达。 结果 TACSTD1和CK19在食管癌组织中的表达量均明显高于食管残端组织（P）。22例患者的血标本检测出TACSTD1和或CK19的高表达。CK19和TACSTD1与患者的肿瘤分化程度高低（P）和T（P）、N（P）分期有密切联系 CK19和TACSTD1在食管癌组织均有特异性的高表达，在食管癌的发生、发展中可能起着重要的作用。CK19和TACSTD1是有价值的食管癌微转移标志物 [关键词]食管癌；微转移；TACSTD1；CK19；QRT-PCRlinical significance of Real-time quantitative PCR detection of esophageal cancer tissue and peripheral blood using CK19 and TACSTD1 as marker Zhang hao,Chen jiajun,Nie ronghua,Hong Bo, Liu rihui ,Zhang yongjun ,Zhan botao (Department of Cardiothoracic Surgery,Xiangfan Central Hospital,Xiangfan,Hubei, 441021,China) [Abstract] Objective To explore clinical significance and Influence on the prognosis of micrometastasis in patients with esophageal cancer by detecting CK19 and TACSTD1 expression in peripheral blood. Methods By using real-time quantitative reverse transcription polymerase chain reaction (QRT-PCR)， the expression of CK19 and TACSTD1 was detected in tumor tissue，stump tissue and peripheral blood from 30 patients with esophageal cancer . Blood from 30 healthy volunteers also were investigated as control groups. Results The expression of CK19 and TACSTD1 in tumor tissue is higher than that in stump tissue（P＜0.01）.The expression of CK19 and or TACSTD1 in peripheral blood of 22 patients is high. CK19 and TACSTD1 are closely linked with the patients degree of tumor differentiation（P）and T（P），N stages（P）.The expression of CK19 and TACSTD1 are specific high in tumor tissue of esophageal cancer，which means are all important in the occurrence and development of esophageal cancer. CK19 and TACSTD1 are good markers for esophageal cancer micrometastasis. [Key words] esophageal cancer;micrometastasis;TACSTD1;CK19;QRT-PCR [通信作者]，