芽孢杆菌属种内基于core基因的亲缘进化分析及其ani的比较-yzxzcom.docVIP

基于全基因组的芽孢杆菌平均核苷酸同源性（ANI）分析 胡桂萍 刘波* 唐唯其 福建省农业科学院农业生物资源研究所，福建省福州市，350003 摘要 采用Jspecies软件分析芽孢杆菌全基因组之间平均核苷酸同源性（ANI）的特征性。结果表明，芽孢杆菌属种间两两菌株间ANI具有明显属种特异性，其中芽孢杆菌科不同属之间的ANI值50%-65%；芽孢杆菌ANI值均低于95%，到%，加权平均数为70.12%；芽孢杆菌亚种ANI值分布范围%到%，。同时基因组中的四核苷酸（Tetra）回归系数与ANI值具有相关性，表现明显种特征性，回归拟合显示两者呈现一元二次方程关系，（R2=0.9812），同时高于70的ANI值与相应的Tetra回归系数呈线性正相关，方程式为（R2=0.8767）。 关键词 芽孢杆菌，系统发育，平均核苷酸同源性，Jspecies Characteristic of average nucleotide identity from Bacillus genus based on the whole genome Hu Gui-ping, Liu Bo*, Tang Wei-qi Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China Abstract: Characteristic of average nucleotide identity (ANI) from Bacillus genus was analyzed based on the whole genome using the soft of Jspecies. The results showed that ANI distribution appeared species specificity. The ANI values computed from different Bacillus genus were below a 70% identity or above a 50% difference in orthologous genes identity. The values from different species were below a identity of 95 with a wide range from 63.77 to 93.81 and the most range from 64.77% to 68.4% with the weighted average of 70.12%，which was recognized as the reference to species identification. And all the ANI value of intra-species were above 95% with the range from 95.5% to 99.77%, and three sorts of the ANI distribution were received which was correlated with the relationship of taxonomic and evolutionary affinities, displaying that the range of ANI from the subspecies lay between the intra-species and species-subsp. Furthermore, Tetra regression index was correlated with the ANI value and displayed the species specificity. Regression fitting analysis showed that the equation of with the correlation coefficient of 0.9812 occurred between them and the ANI values above a identity of 70 were positive correlated with Tetra regression index with the equation of and a 0.8767 of correlation coefficient. Key words: Bacillus, phylogeny, average nucleotide identity, Jspecies 1前言 生物的分类，鉴定及亲缘